AAV GCG-EGFP, a new tool to identify glucagon-secreting ?-cells.
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ABSTRACT:
SUBMITTER: Tuduri E
PROVIDER: S-EPMC6658537 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Publications
AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells.
Tudurí Eva E Glavas Maria M MM Asadi Ali A Baker Robert K RK Ellis Cara E CE Soukhatcheva Galina G Philit Marjolaine M Huynh Frank K FK Johnson James D JD Bruce Verchere C C Kieffer Timothy J TJ
Scientific reports 20190725 1
The study of primary glucagon-secreting α-cells is hampered by their low abundance and scattered distribution in rodent pancreatic islets. We have designed a double-stranded adeno-associated virus containing a rat proglucagon promoter (700 bp) driving enhanced green fluorescent protein (AAV GCG-EGFP), to specifically identify α-cells. The administration of AAV GCG-EGFP by intraperitoneal or intraductal injection led to EGFP expression selectively in the α-cell population. AAV GCG-EGFP delivery t ...[more]
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