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Heavy metals, biomarkers of oxidative stress and changes in sperm function: A case-control study.

ABSTRACT: Background:Heavy metal-induced oxidative stress has been implicated in abnormal sperm functions and male infertility. Objective:Serum and seminal levels of heavy metals and biomarkers of oxidative stress were compared in fertile and infertile men. Materials and Methods:A total of 130 men aged 20-60 yr comprising 30 azoospermic, 50 oligozoospermic, and 50 normozoospermic men were studied. Semen analysis was done by world health organization guidelines, biomarkers of oxidative stress (total antioxidant capacity (TAC), total plasma peroxidase (TPP), oxidative stress index (OSI), vitamin C (vit C) and nitric oxide (NO)) and fructose by colorimetry and serum and seminal heavy metals (zinc (Zn), selenium (Se), cadmium (Cd) and lead (Pb)) by atomic absorption spectrophotometry. Results:Azoospermic and oligozoospermic men had higher serum and seminal peroxides (TPP, p = 0.00), higher serum heavy metals (Zn, Se, Pb, and Cd (p = 0.01)) and lower sperm concentration, %motility, serum and seminal antioxidants (vit C, TAC, NO, GSH (p = 0.01)) compared to normozoospermic men. Higher seminal peroxides (TPP, p = 0.001), heavy metals (Pb and Cd (p = 0.03)) and lower sperm concentration, %motility, and seminal antioxidants (TAC and NO (p = 0.00)) were also observed in azoospermic men compared to oligozoospermic men. Negative correlations were observed between seminal fructose and seminal vit C (r = -0.535, p = 0.015), GSH (r = -0.734, p = 0.000), NO (r = -0.714, p = 0.000), Zn (r = -0.774, p = 0.000) and Se (r = -0.719, p = 0.000) only in azoospermic men. Conclusion:Elevated heavy metal levels, increased lipid peroxidation and antioxidant depletion is associated with abnormal sperm functions in men studied.

SUBMITTER: Chinyere Nsonwu-Anyanwu A

PROVIDER: S-EPMC6661137 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Heavy metals, biomarkers of oxidative stress and changes in sperm function: A case-control study.

Chinyere Nsonwu-Anyanwu Augusta A Raymond Ekong Eworo E Jeremiah Offor Sunday S Francis Awusha Ogar O Chukwuma Orji Oliver O Idiongo Umoh Ediang E Aleruchim Owhorji Jennifer J Rowland Emetonjor Faith F Adanna Opara Usoro Chinyere C

International journal of reproductive biomedicine 20190301 3

<h4>Background</h4>Heavy metal-induced oxidative stress has been implicated in abnormal sperm functions and male infertility.<h4>Objective</h4>Serum and seminal levels of heavy metals and biomarkers of oxidative stress were compared in fertile and infertile men.<h4>Materials and methods</h4>A total of 130 men aged 20-60 yr comprising 30 azoospermic, 50 oligozoospermic, and 50 normozoospermic men were studied. Semen analysis was done by world health organization guidelines, biomarkers of oxidativ ...[more]

PMID: 31435598

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Project description:BackgroundExposure to some toxic metals, such as lead and cadmium, has been associated with increased oxidative stress. However less is known about other metals and metal mixtures, especially in pregnant women who are a vulnerable population.MethodsTo study the relationship between exposure to trace metals and oxidative stress, we analyzed a panel of 17 metals and two oxidative stress biomarkers (8-isoprostane and 8-hydroxydeoxyguanosine [8-OHdG]) in urine samples collected at ~26 weeks gestation from pregnant women in Boston (n = 380). We used linear regression models to calculate percent differences and 95% confidence intervals (CI) in oxidative stress markers for an interquartile range (IQR) increase in each urinary metal with adjustment for other metals. In addition, we applied principal components analysis (PCA) and Bayesian kernel machine regression (BKMR), to examine cumulative effects (within correlated groups of exposures as well as overall) and interactions.ResultsWe estimated 109% (95% CI: 47, 198) higher 8-isoprostane and 71% (95% CI: 45, 102) higher 8-OHdG with an IQR increase in urinary selenium (Se). We also estimated higher 8-isoprostane (47%, 95% CI: 20.5, 79.4) and 8-OHdG (15.3%, 95% CI: 5.09, 26.5) in association with urinary copper (Cu). In our PCA, we observed higher 8-isoprostane levels in association with the "essential" PC (highly loaded by Cu, Se, and Zinc). In BKMR analyses, we also estimated higher levels of both oxidative stress biomarkers with increasing Se and Cu as well as increasing levels of both oxidative stress biomarkers in association with cumulative concentrations of urinary trace metals.ConclusionWe observed higher 8-isoprostane and 8-OHdG levels in association with urinary trace metals and elements, particularly Se and Cu, in linear models and using mixtures approaches. Additionally, increasing cumulative exposure to urinary trace metals was associated with higher levels of both oxidative stress biomarkers. The beneficial effects of these compounds should be carefully questioned.

Dataset Information

Heavy metals, biomarkers of oxidative stress and changes in sperm function: A case-control study.

Publications

Heavy metals, biomarkers of oxidative stress and changes in sperm function: A case-control study.

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