Ontology highlight
ABSTRACT:
SUBMITTER: Pincus D
PROVIDER: S-EPMC6662207 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Pincus David D Pandey Jai P JP Feder Zoë A ZA Creixell Pau P Resnekov Orna O Reynolds Kimberly A KA
Science signaling 20181106 555
Phosphoregulation, in which the addition of a negatively charged phosphate group modulates protein activity, enables dynamic cellular responses. To understand how new phosphoregulation might be acquired, we mutationally scanned the surface of a prototypical yeast kinase (Kss1) to identify potential regulatory sites. The data revealed a set of spatially distributed "hotspots" that might have coevolved with the active site and preferentially modulated kinase activity. By engineering simple consens ...[more]