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Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.

ABSTRACT: Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low-resolution diffracting samples or crystals with twinning. Thanks to Tb-Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches.

SUBMITTER: Engilberge S

PROVIDER: S-EPMC6662991 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.

Engilberge Sylvain S Wagner Tristan T Santoni Gianluca G Breyton Cécile C Shima Seigo S Franzetti Bruno B Riobé Francois F Maury Olivier O Girard Eric E

Journal of applied crystallography 20190628 Pt 4

Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such a ...[more]

PMID: 31396026

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