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Real-Time PCR Assay for Differentiation of Typhoidal and Nontyphoidal Salmonella.


ABSTRACT: Rapid and accurate differentiation of Salmonella spp. causing enteric fever from nontyphoidal Salmonella is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly on crude DNA extracts from bacterial colonies for the rapid identification of typhoidal and nontyphoidal Salmonella.

SUBMITTER: Nair S 

PROVIDER: S-EPMC6663909 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Real-Time PCR Assay for Differentiation of Typhoidal and Nontyphoidal <i>Salmonella</i>.

Nair Satheesh S   Patel Vineet V   Hickey Tadgh T   Maguire Clare C   Greig David R DR   Lee Winnie W   Godbole Gauri G   Grant Kathie K   Chattaway Marie Anne MA  

Journal of clinical microbiology 20190726 8


Rapid and accurate differentiation of <i>Salmonella</i> spp. causing enteric fever from nontyphoidal <i>Salmonella</i> is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly  ...[more]

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