Knockdown of estrogen receptor ? increases proliferation and affects the transcriptome of endometrial adenocarcinoma cells.

ABSTRACT: BACKGROUND:Estrogen receptor ? (ER?) has been repeatedly suggested to play important roles in hormone-dependent cancer like in tumors of the breast, ovary or prostate. In this study, we intended to further elucidate its role in endometrial cancer. METHODS:For this purpose, we knocked down ER? expression in two endometrial cancer cell lines, the ER?-negative/ER?-positive line HEC-1A and the ER?/?-positive cell line RL95/2, by means of siRNA transfection. Cell proliferation after transfection was assessed using the fluorescent CTB Assay (Promega). In order to elucidate possible molecular mechanisms which might underlie the effect on proliferation, we performed transcriptome analyses by means of human Affymetrix Human Gene Chip 2.0. Additionally, we treated the employed cell lines with different ER? modulators to examine their effect on proliferation. RESULTS:siRNA-mediated knockdown of ER? significantly increased proliferation of both endometrial cancer cell lines. In HEC-1A cells, proliferation was significantly increased 4, 5 and 6?days after transfection, with a maximum of about 1.7-fold (p?

SUBMITTER: Treeck O 

PROVIDER: S-EPMC6664594 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature