Dataset Information

BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation.

ABSTRACT:

Background

Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from Bothrops jararacussu on MCF7, SKBR3, and MDAMB231 breast cancer cell lines.

Methods

BthTX-I cytotoxicity was determined via MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay. Cell death was measured by a hypotonic fluorescent solution method, annexin-V-FITC/propidium iodide staining and by apoptotic/autophagic protein expression. Cancer stem cells (CSCs) were quantified by flow cytometry using anti-CD24-FITC and anti-CD44-APC antibodies and propidium iodide.

Results

BthTX-I at 102 µg/mL induced cell death in all cell lines. The toxin induced apoptosis in MCF7, SKBR3, and MDAMB231 in a dose-dependent manner, as confirmed by the increasing number of hypodiploid nuclei. Expression of pro-caspase 3, pro-caspase 8 and Beclin-1 proteins were increased, while the level of the antiapoptotic protein Bcl-2 was diminished in MCF7 cells. BthTX-I changed the staining pattern of CSCs in MDAMB231 cells by increasing expression of CD24 receptors, which mediated cell death.

Conclusions

BthTX-I induces apoptosis and autophagy in all breast cancer cell lines tested and also reduces CSCs subpopulation, which makes it a promising therapeutic alternative for breast cancer.

SUBMITTER: Bezerra PHA

PROVIDER: S-EPMC6665320 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

BthTX-I from <i>Bothrops jararacussu</i> induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation.

Bezerra Patrícia Heloise Alves PHA Ferreira Isadora Marques IM Franceschi Beatriz Tinoco BT Bianchini Francine F Ambrósio Luciana L Cintra Adélia Cristina O ACO Sampaio Suely Vilela SV de Castro Fabíola Attié FA Torqueti Maria Regina MR

The journal of venomous animals and toxins including tropical diseases 20190729

<h4>Background</h4>Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from <i>Bothrops jararacussu</i> on MCF7, SKBR3, and MDAMB231 breast cancer cell lines.<h4>Methods</h4>BthTX-I cytotoxicity was determined via MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay. Cell death was measured by a hypotonic fluorescent solut ...[more]

PMID: 31384244

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