Ontology highlight
ABSTRACT:
SUBMITTER: Duan J
PROVIDER: S-EPMC6667192 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Duan Jialei J Li Boxun B Bhakta Minoti M Xie Shiqi S Zhou Pei P Munshi Nikhil V NV Hon Gary C GC
Cell reports 20190601 12
Ectopic expression of transcription factors (TFs) can reprogram cell state. However, because of the large combinatorial space of possible TF cocktails, it remains difficult to identify TFs that reprogram specific cell types. Here, we develop Reprogram-Seq to experimentally screen thousands of TF cocktails for reprogramming performance. Reprogram-Seq leverages organ-specific cell-atlas data with single-cell perturbation and computational analysis to predict, evaluate, and optimize TF combinations ...[more]