Genetically diverse Pseudomonas aeruginosa populations display similar transcriptomic profiles in a cystic fibrosis explanted lung.
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ABSTRACT:
SUBMITTER: Kordes A
PROVIDER: S-EPMC6667473 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
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Genetically diverse Pseudomonas aeruginosa populations display similar transcriptomic profiles in a cystic fibrosis explanted lung.
Kordes Adrian A Preusse Matthias M Willger Sven D SD Braubach Peter P Jonigk Danny D Haverich Axel A Warnecke Gregor G Häussler Susanne S
Nature communications 20190730 1
Previous studies have demonstrated substantial genetic diversification of Pseudomonas aeruginosa across sub-compartments in cystic fibrosis (CF) lungs. Here, we isolate P. aeruginosa from five different sampling areas in the upper and lower airways of an explanted CF lung, analyze ex vivo transcriptional profiles by RNA-seq, and use colony re-sequencing and deep population sequencing to determine the genetic diversity within and across the various sub-compartments. We find that, despite genetic ...[more]
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