Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro.
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ABSTRACT: Background
Reported efficacy of platelet-rich plasma (PRP) in regenerative medicine is contradictory. We validated the effects of PRP on proliferation of canine bone marrow-derived multipotent mesenchymal stromal cells (K9BMMSCs) in vitro. PRP was extracted from blood of six dogs with osteoarthritis. K9BMMSCs were established from bone marrow and characterized for CD90 and CD19 expression by immunocytochemistry. Effects of PRP concentrations on viability of matching autologous K9BMMSCs were validated using MTS assay.Results
Positive CD90 and negative CD19 expression confirmed MSC origin. PRP at 40% volume/volume concentration increased, while PRP at 80 and 100% v/v concentrations suppressed viability of tested K9BMMSCs.Conclusion
PRP concentration plays an important role in K9BMMSCs viability, which could affect tissue repairs in vivo.
SUBMITTER: Pandey S
PROVIDER: S-EPMC6668135 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Publications
Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro.
Pandey Sony S Hickey Dawn U DU Drum Marti M Millis Darryl L DL Cekanova Maria M
BMC veterinary research 20190730 1
<h4>Background</h4>Reported efficacy of platelet-rich plasma (PRP) in regenerative medicine is contradictory. We validated the effects of PRP on proliferation of canine bone marrow-derived multipotent mesenchymal stromal cells (K9BMMSCs) in vitro. PRP was extracted from blood of six dogs with osteoarthritis. K9BMMSCs were established from bone marrow and characterized for CD90 and CD19 expression by immunocytochemistry. Effects of PRP concentrations on viability of matching autologous K9BMMSCs w ...[more]
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