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Fabrication of 2D Hetero-Complexes With Nucleic-Acid-Base Adenine and Fatty-Acid Stearic Acid at Liquid/Solid Interface.

ABSTRACT: Designing and fabricating hetero-complexes composed of organic and biological compounds had become an exciting area referring to biological recognition, molecular devices etc. Here, hydrogen-bonded complex of nucleic-acid-base (adenine, A) and fatty-acid (stearic acid, SA) was designed, fabricated and investigated at liquid/solid interface. The interesting striped-shaped structure composed of SA-A-SA trimers was formed after introducing adenine molecules. Meanwhile, the primary lamella-shape characteristic of the assembly of SA molecules was kept because of the collaboration of non-covalent interactions of molecule-molecule and molecule-substrate. With a series of experimental characterization and theoretical simulation, the origination of the as-prepared 2D hetero-complexes was gradually exhibited from the assembled structures of two building blocks of stearic acid and adenine. Our study provides a blueprint for designing additional multi-component complexes based on the existing molecular assembled architectures.

SUBMITTER: Zhao H

PROVIDER: S-EPMC6669938 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Digital Light Processing (DLP) stereolithography (SLA) as a high-resolution 3D printing process offers a low-cost alternative for prototyping of microfluidic geometries, compared to traditional clean-room and workshop-based methods. Here, we investigate DLP-SLA printing performance for the production of micro-chamber chip geometries suitable for Polymerase Chain Reaction (PCR), a key process in molecular diagnostics to amplify nucleic acid sequences. A DLP-SLA fabrication protocol for printed micro-chamber devices with monolithic micro-channels is developed and evaluated. Printed devices were post-processed with ultraviolet (UV) light and solvent baths to reduce PCR inhibiting residuals and further treated with silane coupling agents to passivate the surface, thereby limiting biomolecular adsorption occurences during the reaction. The printed devices were evaluated on a purpose-built infrared (IR) mediated PCR thermocycler. Amplification of 75 base pair long target sequences from genomic DNA templates on fluorosilane and glass modified chips produced amplicons consistent with the control reactions, unlike the non-silanized chips that produced faint or no amplicon. The results indicated good functionality of the IR thermocycler and good PCR compatibility of the printed and silanized SLA polymer. Based on the proposed methods, various microfluidic designs and ideas can be validated in-house at negligible costs without the requirement of tool manufacturing and workshop or clean-room access. Additionally, the versatile chemistry of 3D printing resins enables customised surface properties adding significant value to the printed prototypes. Considering the low setup and unit cost, design flexibility and flexible resin chemistries, DLP-SLA is anticipated to play a key role in future prototyping of microfluidics, particularly in the fields of research biology and molecular diagnostics. From a system point-of-view, the proposed method of thermocycling shows promise for portability and modular integration of funcitonalitites for diagnostic or research applications that utilize nucleic acid amplification technology.

Dataset Information

Fabrication of 2D Hetero-Complexes With Nucleic-Acid-Base Adenine and Fatty-Acid Stearic Acid at Liquid/Solid Interface.

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