Dataset Information

Changes in microparticle profiles by vitamin D receptor activation in chronic kidney disease - a randomized trial.

ABSTRACT:

Background

Microparticles (MPs) are biomarkers and mediators of disease through their expression of surface receptors, reflecting activation or stress in their parent cells. Endothelial markers, ICAM-1 and VCAM-1, are implicated in atherosclerosis and associated with cardiovascular risk. Chronic kidney disease (CKD) patients have endothelial dysfunction and high levels of endothelial derived MPs. Vitamin D treatment has been reported to ameliorate endothelial function in CKD patients. We aimed to examine cell specific MP profiles and concentrations of MPs expressing the atherosclerotic markers ICAM-1 and VCAM-1 after treatment with paricalcitol in patients with CKD stage 3-4.

Methods

Sub-study of the previously reported SOLID trial where 36 patients were randomly assigned to placebo, 1 or 2 μg paricalcitol, for 12 weeks. MPs were measured by flow cytometry after labelling with antibodies against endothelial (CD62E), platelet (CD62P, CD41, CD154) leukocyte (CD45) and vascular (CD54, CD106) markers.

Results

Patients had a mean age of 65 years with a mean eGFR of 40 mL/min/1.73m². Concentrations of ICAM-1 positive MPs were significantly reduced by treatment (repeated measures ANOVA p = 0.04). Repeated measures MANOVA of concentrations of endothelial, platelet and leukocyte MPs showed sustained levels in the 2 μg treatment group (p = 0.85) but a decline in the 1 μg (p = 0.04) and placebo groups (p = 0.005).

Conclusions

Treatment with paricalcitol reduces concentrations of ICAM-1 positive MPs. This is accompanied by sustained concentrations of all cell specific MPs in the 2 μg group, and decreasing concentrations in the other groups, possibly due to a more healthy and reactive endothelium with paricalcitol treatment.

SUBMITTER: Lundwall K

PROVIDER: S-EPMC6670162 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Publications

Changes in microparticle profiles by vitamin D receptor activation in chronic kidney disease - a randomized trial.

Lundwall Kristina K Mörtberg Josefin J Mobarrez Fariborz F Jacobson Stefan H SH Jörneskog Gun G Spaak Jonas J

BMC nephrology 20190801 1

<h4>Background</h4>Microparticles (MPs) are biomarkers and mediators of disease through their expression of surface receptors, reflecting activation or stress in their parent cells. Endothelial markers, ICAM-1 and VCAM-1, are implicated in atherosclerosis and associated with cardiovascular risk. Chronic kidney disease (CKD) patients have endothelial dysfunction and high levels of endothelial derived MPs. Vitamin D treatment has been reported to ameliorate endothelial function in CKD patients. We ...[more]

PMID: 31370809

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Dataset Information

Changes in microparticle profiles by vitamin D receptor activation in chronic kidney disease - a randomized trial.

Background

Methods

Results

Conclusions

Publications

Changes in microparticle profiles by vitamin D receptor activation in chronic kidney disease - a randomized trial.

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