Ontology highlight
ABSTRACT:
SUBMITTER: Demidov D
PROVIDER: S-EPMC6671561 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Demidov Dmitri D Heckmann Stefan S Weiss Oda O Rutten Twan T Dvořák Tomaštíková Eva E Kuhlmann Markus M Scholl Patrick P Municio Celia Maria CM Lermontova Inna I Houben Andreas A
Frontiers in plant science 20190725
Several histone variants are posttranslationally phosphorylated. Little is known about phosphorylation of the centromere-specific histone 3 (CENH3) variant in plants. We show that CENH3 of <i>Arabidopsis thaliana</i> is phosphorylated <i>in vitro</i> by Aurora3, predominantly at serine 65. Interaction of Aurora3 and CENH3 was found by immunoprecipitation (IP) in <i>A. thaliana</i> and by bimolecular fluorescence complementation. Western blotting with an anti-CENH3 pS65 antibody showed that CENH3 ...[more]