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Aspergillus oryzae spore germination is enhanced by non-thermal atmospheric pressure plasma.

ABSTRACT: Poor and unstable culture growth following isolation presents a technical barrier to the efficient application of beneficial microorganisms in the food industry. Non-thermal atmospheric pressure plasma is an effective tool that could overcome this barrier. The objective of this study was to investigate the potential of plasma to enhance spore germination, the initial step in fungal colonization, using Aspergillus oryzae, a beneficial filamentous fungus used in the fermentation industry. Treating fungal spores in background solutions of phosphate buffered saline (PBS) and potato dextrose broth (PDB) with micro dielectric barrier discharge plasma using nitrogen gas for 2 and 5?min, respectively, significantly increased the germination percentage. Spore swelling, the first step in germination, was accelerated following plasma treatment, indicating that plasma may be involved in loosening the spore surface. Plasma treatment depolarized spore membranes, elevated intracellular Ca²⁺ levels, and activated mpkA, a MAP kinase, and the transcription of several germination-associated genes. Our results suggest that plasma enhances fungal spore germination by stimulating spore swelling, depolarizing the cell membrane, and activating calcium and MAPK signaling.

SUBMITTER: Veerana M

PROVIDER: S-EPMC6673704 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Aspergillus oryzae spore germination is enhanced by non-thermal atmospheric pressure plasma.

Veerana Mayura M Lim Jun-Sup JS Choi Eun-Ha EH Park Gyungsoon G

Scientific reports 20190801 1

Poor and unstable culture growth following isolation presents a technical barrier to the efficient application of beneficial microorganisms in the food industry. Non-thermal atmospheric pressure plasma is an effective tool that could overcome this barrier. The objective of this study was to investigate the potential of plasma to enhance spore germination, the initial step in fungal colonization, using Aspergillus oryzae, a beneficial filamentous fungus used in the fermentation industry. Treating ...[more]

PMID: 31371801

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Project description:The heat convection generated by micro filaments of a self-organized non-thermal atmospheric pressure plasma jet in Ar is characterized by employing laser schlieren deflectometry (LSD). It is demonstrated as a proof of principle, that the spatial and temporal changes of the refractive index n in the optical beam path related to the neutral gas temperature of the plasma jet can be monitored and evaluated simultaneously. The refraction of a laser beam in a high gradient field of n(r) with cylindrical symmetry is given for a general real refraction index profile. However, the usually applied Abel approach represents an ill-posed problem and in particular for this plasma configuration. A simple analytical model is proposed in order to minimize the statistical error. Based on that, the temperature profile, specifically the absolute temperature in the filament core, the FWHM, and the frequencies of the collective filament dynamics are obtained for non-stationary conditions. For a gas temperature of 700 K inside the filament, the presented model predicts maximum deflection angles of the laser beam of 0.3 mrad which is in accordance to the experimental results obtained with LSD. Furthermore, the experimentally obtained FWHM of the temperature profile produced by the filament at the end of capillary is (1.5 ± 0.2) mm, which is about 10 times wider than the visual radius of the filament. The obtained maximum temperature in the effluent is (450 ± 30) K and is in consistence with results of other techniques. The study demonstrates that LSD represents a useful low-cost method for monitoring the spatiotemporal behaviour of microdischarges and allows to uncover their dynamic characteristics, e.g., the temperature profile even for challenging diagnostic conditions such as moving thin discharge filaments. The method is not restricted to the miniaturized and self-organized plasma studied here. Instead, it can be readily applied to other configurations that produce measurable gradients of refractive index by local gas heating and opens new diagnostics prospects particularly for microplasmas.

Dataset Information

Aspergillus oryzae spore germination is enhanced by non-thermal atmospheric pressure plasma.

Publications

Aspergillus oryzae spore germination is enhanced by non-thermal atmospheric pressure plasma.

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