Browse
Submit Data
Databases
API
Help

Dataset Information

30 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A miniaturized spectrometer for NMR relaxometry under extreme conditions.

ABSTRACT: With the advent of integrated electronics, microfabrication and novel chemistry, NMR (Nuclear Magnetic Resonance) methods, embodied in miniaturized spectrometers, have found profound uses in recent years that are beyond their conventional niche. In this work, we extend NMR relaxometry on a minute sample below 20??L to challenging environment of 150?°C in temperature and 900?bar in pressure. Combined with a single-board NMR spectrometer, we further demonstrate multidimensional NMR relaxometries capable of resolving compositions of complex fluids. The confluence of HTHP (high-pressure high-temperature) capability, minimal sample volume, and reduced sensor envelop and power budget creates a new class of mobile NMR platforms, bringing the powerful analytical toolkit in a miniaturized footprint to extreme operating conditions.

SUBMITTER: Tang Y

PROVIDER: S-EPMC6673705 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

A miniaturized spectrometer for NMR relaxometry under extreme conditions.

Tang Yiqiao Y McCowan David D Song Yi-Qiao YQ

Scientific reports 20190801 1

With the advent of integrated electronics, microfabrication and novel chemistry, NMR (Nuclear Magnetic Resonance) methods, embodied in miniaturized spectrometers, have found profound uses in recent years that are beyond their conventional niche. In this work, we extend NMR relaxometry on a minute sample below 20 μL to challenging environment of 150 °C in temperature and 900 bar in pressure. Combined with a single-board NMR spectrometer, we further demonstrate multidimensional NMR relaxometries c ...[more]

PMID: 31371756

Similar Datasets

Crenarchaeal biofilm formation under extreme conditions.

Project description:BackgroundBiofilm formation has been studied in much detail for a variety of bacterial species, as it plays a major role in the pathogenicity of bacteria. However, only limited information is available for the development of archaeal communities that are frequently found in many natural environments.MethodologyWe have analyzed biofilm formation in three closely related hyperthermophilic crenarchaeotes: Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii. We established a microtitre plate assay adapted to high temperatures to determine how pH and temperature influence biofilm formation in these organisms. Biofilm analysis by confocal laser scanning microscopy demonstrated that the three strains form very different communities ranging from simple carpet-like structures in S. solfataricus to high density tower-like structures in S. acidocaldarius in static systems. Lectin staining indicated that all three strains produced extracellular polysaccharides containing glucose, galactose, mannose and N-acetylglucosamine once biofilm formation was initiated. While flagella mutants had no phenotype in two days old static biofilms of S. solfataricus, a UV-induced pili deletion mutant showed decreased attachment of cells.ConclusionThe study gives first insights into formation and development of crenarchaeal biofilms in extreme environments.

| S-EPMC2991349 | biostudies-literature

Miniaturized Near-Infrared (MicroNIR) Spectrometer in Plastic Waste Sorting.

Project description:Valorisation of the urban plastic waste in high-quality recyclates is an imperative challenge in the new paradigm of the circular economy. In this scenario, a key role in the improvement of the recycling process is exerted by the optimization of waste sorting. In spite of the enormous developments achieved in the field of automated sorting systems, the quest for the reduction of cross-contamination of incompatible polymers as well as a rapid and punctual sorting of the unmatched polymers has not been sufficiently developed. In this paper, we demonstrate that a miniaturized handheld near-infrared (NIR) spectrometer can be used to successfully fingerprint and classify different plastic polymers. The investigated urban plastic waste comprised polyethylene (PE), polypropylene (PP), poly(vinyl chloride) (PVC), poly(ethylene terephthalate) (PET), and poly(styrene) (PS), collected directly in a recycling plastic waste plant, without any kind of sample washing or treatment. The application of unsupervised and supervised chemometric tools such as principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) on the NIR dataset resulted in a complete classification of the polymer classes. In addition, several kinds of PET (clear, blue, coloured, opaque, and boxes) were correctly classified as PET class, and PE samples with different branching degrees were properly separated.

| S-EPMC6747759 | biostudies-literature

Limitations of microbial iron reduction under extreme conditions.

Project description:Microbial iron reduction is a widespread and ancient metabolism on Earth, and may plausibly support microbial life on Mars and beyond. Yet, the extreme limits of this metabolism are yet to be defined. To investigate this, we surveyed the recorded limits to microbial iron reduction in a wide range of characterized iron-reducing microorganisms (n = 141), with a focus on pH and temperature. We then calculated Gibbs free energy of common microbially mediated iron reduction reactions across the pH-temperature habitability space to identify thermodynamic limits. Comparing predicted and observed limits, we show that microbial iron reduction is generally reported at extremes of pH or temperature alone, but not when these extremes are combined (with the exception of a small number of acidophilic hyperthermophiles). These patterns leave thermodynamically favourable combinations of pH and temperature apparently unoccupied. The empty spaces could be explained by experimental bias, but they could also be explained by energetic and biochemical limits to iron reduction at combined extremes. Our data allow for a review of our current understanding of the limits to microbial iron reduction at extremes and provide a basis to test more general hypotheses about the extent to which biochemistry establishes the limits to life.

| S-EPMC9629499 | biostudies-literature

Lichen metabolites produced under extreme conditions

Project description:Non-target metabolomics of lichens exposed to extreme environmental conditions such as pressure and ultraviolet light

2023-07-04 | MSV000092339 | MassIVE

Extreme extensibility of copper foil under compound forming conditions.

Project description:A copper foil with an extreme extensibility up to 43,684% was obtained without any intermediate annealing by means of asynchronous rolling with high tension. It was found that under the combination of compression, shearing and tension, the copper foil represents a wonderful phenomenon. As the reduction increases, the specimen hardness increases up to a peak value 138 HV0.05 when the foil thickness rolled to around 100??m, and then it decreases down to 78 HV0.05 when the foil thickness rolled to the final size 19??m. It tells us that the strain-softening effect occurs when the foil thickness is rolled down to a threshold level. The experimental results bring us some fresh ideas different with the traditional understanding on the strain-hardening mechanism of metals, which provides an experimental basis to establish the forming mechanism of the thin foil.

| S-EPMC6506452 | biostudies-literature

Solutions for Critical Raw Materials under Extreme Conditions: A Review.

Project description:In Europe, many technologies with high socio-economic benefits face materials requirements that are often affected by demand-supply disruption. This paper offers an overview of critical raw materials in high value alloys and metal-matrix composites used in critical applications, such as energy, transportation and machinery manufacturing associated with extreme working conditions in terms of temperature, loading, friction, wear and corrosion. The goal is to provide perspectives about the reduction and/or substitution of selected critical raw materials: Co, W, Cr, Nb and Mg.

| S-EPMC5503360 | biostudies-other

Anomalous mechanical behavior of nanocrystalline binary alloys under extreme conditions.

Project description:Fundamentally, material flow stress increases exponentially at deformation rates exceeding, typically, ~103?s-1, resulting in brittle failure. The origin of such behavior derives from the dislocation motion causing non-Arrhenius deformation at higher strain rates due to drag forces from phonon interactions. Here, we discover that this assumption is prevented from manifesting when microstructural length is stabilized at an extremely fine size (nanoscale regime). This divergent strain-rate-insensitive behavior is attributed to a unique microstructure that alters the average dislocation velocity, and distance traveled, preventing/delaying dislocation interaction with phonons until higher strain rates than observed in known systems; thus enabling constant flow-stress response even at extreme conditions. Previously, these extreme loading conditions were unattainable in nanocrystalline materials due to thermal and mechanical instability of their microstructures; thus, these anomalies have never been observed in any other material. Finally, the unique stability leads to high-temperature strength maintained up to 80% of the melting point (~1356?K).

| S-EPMC6043485 | biostudies-literature

Anomalous Optical Properties of Citrazinic Acid under Extreme pH Conditions.

Project description:Citrazinic acid (CZA) is a weakly fluorescent molecular compound whose optical properties are dependent on aggregation states and chemical environment. This molecule and its derivatives have been recently identified as the source of the intense blue emission of carbon dots obtained from citric acid with a nitrogen source, such as ammonia or urea. Citrazinic acid has a strong tendency to aggregate and form tautomers whose optical properties are largely unexplored. At extreme acidic and basic pH values, we have observed an "anomalous" optical response of citrazinic acid, attributed to the formation of aggregates from the tautomers. We have characterized the molecule, both at pH = 1 and 14, using UV-vis, NMR, steady-state, and time-resolved fluorescence spectroscopy. At extremely low pH values, the protonation causes luminescence quenching and the appearance of new emissions. On the contrary, high pH values are responsible for deprotonation and splitting of the excitation spectra.

| S-EPMC7241015 | biostudies-literature

An archaeal tRNA-synthetase complex that enhances aminoacylation under extreme conditions.

Project description:Aminoacyl-tRNA synthetases (aaRSs) play an integral role in protein synthesis, functioning to attach the correct amino acid with its cognate tRNA molecule. AaRSs are known to associate into higher-order multi-aminoacyl-tRNA synthetase complexes (MSC) involved in archaeal and eukaryotic translation, although the precise biological role remains largely unknown. To gain further insights into archaeal MSCs, possible protein-protein interactions with the atypical Methanothermobacter thermautotrophicus seryl-tRNA synthetase (MtSerRS) were investigated. Yeast two-hybrid analysis revealed arginyl-tRNA synthetase (MtArgRS) as an interacting partner of MtSerRS. Surface plasmon resonance confirmed stable complex formation, with a dissociation constant (K(D)) of 250 nM. Formation of the MtSerRS·MtArgRS complex was further supported by the ability of GST-MtArgRS to co-purify MtSerRS and by coelution of the two enzymes during gel filtration chromatography. The MtSerRS·MtArgRS complex also contained tRNA(Arg), consistent with the existence of a stable ribonucleoprotein complex active in aminoacylation. Steady-state kinetic analyses revealed that addition of MtArgRS to MtSerRS led to an almost 4-fold increase in the catalytic efficiency of serine attachment to tRNA, but had no effect on the activity of MtArgRS. Further, the most pronounced improvements in the aminoacylation activity of MtSerRS induced by MtArgRS were observed under conditions of elevated temperature and osmolarity. These data indicate that formation of a complex between MtSerRS and MtArgRS provides a means by which methanogenic archaea can optimize an early step in translation under a wide range of extreme environmental conditions.

| S-EPMC3030346 | biostudies-other

Microbial mediated formation of Fe-carbonate minerals under extreme acidic conditions.

Project description:Discovery of Fe-carbonate precipitation in Rio Tinto, a shallow river with very acidic waters, situated in Huelva, South-western Spain, adds a new dimension to our understanding of carbonate formation. Sediment samples from this low-pH system indicate that carbonates are formed in physico-chemical conditions ranging from acid to neutral pH. Evidence for microbial mediation is observed in secondary electron images (Fig. 1), which reveal rod-shaped bacteria embedded in the surface of siderite nanocrystals. The formation of carbonates in Rio Tinto is related to the microbial reduction of ferric iron coupled to the oxidation of organic compounds. Herein, we demonstrate for the first time, that Acidiphilium sp. PM, an iron-reducing bacterium isolated from Rio Tinto, mediates the precipitation of siderite (FeCO3) under acidic conditions and at a low temperature (30°C). We describe nucleation of siderite on nanoglobules in intimate association with the bacteria cell surface. This study has major implications for understanding carbonate formation on the ancient Earth or extraterrestrial planets.

| S-EPMC3996482 | biostudies-other

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data