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Influenza-Mediated Lung Infection Models.


ABSTRACT: Laboratory rodent influenza infection models have been and continue to be a critical tool for understanding virus-host interactions during infection. The incidence of seasonal influenza infections coupled with the need for novel therapeutics and universal vaccines highlights the need to uncover novel mechanisms of pathogenesis and protection. Mouse models are extremely useful for the evaluation of influenza vaccines and provide an invaluable tool to probe the immune response. This chapter describes the technique of intranasal inoculation of male C57BL/6J mice with an H1N1 strain of influenza (A/Puerto Rico/8/1934) and methods for assessing the optimum dose for infection, viral titers in lung tissue, and severity of disease.

SUBMITTER: McGee CE 

PROVIDER: S-EPMC6675590 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Influenza-Mediated Lung Infection Models.

McGee Charles E CE   Sample Christopher J CJ   Kilburg-Basnyat Brita B   Gabor Kristin A KA   Fessler Michael B MB   Gowdy Kymberly M KM  

Methods in molecular biology (Clifton, N.J.) 20190101


Laboratory rodent influenza infection models have been and continue to be a critical tool for understanding virus-host interactions during infection. The incidence of seasonal influenza infections coupled with the need for novel therapeutics and universal vaccines highlights the need to uncover novel mechanisms of pathogenesis and protection. Mouse models are extremely useful for the evaluation of influenza vaccines and provide an invaluable tool to probe the immune response. This chapter descri  ...[more]

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