Dataset Information

Aging-associated microstructural deterioration of vertebra in zebrafish.

ABSTRACT: Zebrafish, a small teleost fish, is currently emerging as an animal model of local and systemic aging. In this study, we assessed age-related degenerative changes in the vertebral bone of zebrafish (3-12?month-post-fertilisation [mpf]) using micro-CT scanning. The bone volume (BV) of the trabecular bone in the male and female fish peaked at 6?mpf and reduced with age. In contrast to BV, bone mineral density and tissue volume did not change after 6?mpf, implying that the total mineral volume in the trabecular area remains unchanged, retaining the strength of vertebra. In addition, we performed micro-structural analysis of the trabecular thickness, trabecular number, and star volume of the tissue space and trabeculae, and found that the size of the trabecular bone reduced with age. Furthermore, aged zebrafish (45?mpf) exhibited ectopic ossification inside or outside of their vertebrae. In summary, we analysed bone structural parameters in adult zebrafish vertebra, which are also used in humans, and demonstrated that aged zebrafish have deteriorated microarchitecture (trabecular thickness and number, tissue space star volume and trabecular star volume) with reduction of trabecular bones, similar to that observed during aging in humans. Zebrafish can be utilised as an animal model to understand the pathology of human bone aging, and the discovery of new therapeutic agents against age-related osteoporosis.

SUBMITTER: Monma Y

PROVIDER: S-EPMC6676153 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Aging-associated microstructural deterioration of vertebra in zebrafish.

Monma Yasuyuki Y Shimada Yasuhito Y Nakayama Hiroko H Zang Liqing L Nishimura Norihiro N Tanaka Toshio T

Bone reports 20190717

Zebrafish, a small teleost fish, is currently emerging as an animal model of local and systemic aging. In this study, we assessed age-related degenerative changes in the vertebral bone of zebrafish (3-12 month-post-fertilisation [mpf]) using micro-CT scanning. The bone volume (BV) of the trabecular bone in the male and female fish peaked at 6 mpf and reduced with age. In contrast to BV, bone mineral density and tissue volume did not change after 6 mpf, implying that the total mineral volume in t ...[more]

PMID: 31388517

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Project description:The likelihood of experiencing an osteoporotic fracture of one or more vertebral bodies increases with age, and this increase is not solely due to sex steroid deficiency. For the purpose of assessing the effectiveness of novel therapeutic strategies in the prevention of vertebral fractures among the elderly, we hypothesized that the BALB/c mouse model of aging phenocopies the age-related decrease in human VB strength. To test this hypothesis, we assessed the age-related changes in trabecular architecture of the L6 VB, with respect to those in the distal femur metaphysis, between 6-mo. (young adulthood, n = 20/sex) and 20-mo. of age (old age, n = 18/sex) and then determined how well the architectural characteristics, volumetric bone mineral density (vBMD), and predicted failure force from μCT-derived finite element analysis (μFEA) with linear elastic failure criteria explained the age-related variance in VB strength, which was the ultimate force during quasi-static loading of the VB in compression. While there was a pronounced age-related deterioration in trabecular architecture in the distal femur metaphysis of female and male BALB/c mice, the decrease in trabecular bone volume fraction and trabecular number between 6-mo. and 20-mo. of age occurred in male mice, but not in female mice. As such, the VB strength was lower with age in males only. Nonetheless, BV/TV and volumetric bone mineral density (vBMD) positively correlated with the ultimate compressive force of the L6 VB for both females and males. Whether using a fixed homogeneous distribution of tissue modulus (Et = 18 GPa) or a heterogeneous distribution of Et based on a positive relationship with TMD, the predicted failure force of the VB was not independent of age, thereby suggesting linear μFEA may not be a suitable replacement for mechanical-based measurements of strength with respect to age-related changes. Overall, the BALB/c mouse model of aging mimics the age-related in decline in human VB strength when comparing 6-mo. and 20-mo. old male mice. The decrease in VB strength in female mice may occur over a different age range.

Dataset Information

Aging-associated microstructural deterioration of vertebra in zebrafish.

Publications

Aging-associated microstructural deterioration of vertebra in zebrafish.

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