ROR2 modulates neuropathic pain via phosphorylation of NMDA receptor subunit GluN2B in rats.

ABSTRACT:

Background

Neuropathic pain, a type of chronic pain as a result of direct central or peripheral nerve damage, is associated with significant quality of life and functional impairment. Its underlying mechanisms remain unclear. We investigated whether ROR2, a member of the receptor tyrosine kinase-like orphan receptor (ROR) family, participates in modulation of neuropathic pain.

Methods

Thermal hyperalgesia and mechanical allodynia were measured using radiant heat and von Frey filament testing. Immunofluorescence staining was used to detect expression of ROR2 in neuronal nuclei. Fos expression was determined by immunocytochemistry. Phosphorylation status was detected by western blot and immunoprecipitation. Small interfering RNA was used to knock down ROR2 expression.

Resul

SUBMITTER: Zhou XL 

PROVIDER: S-EPMC6676246 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature