Ontology highlight
ABSTRACT:
SUBMITTER: Brown JR
PROVIDER: S-EPMC6676502 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Biology methods & protocols 20190801 1
The neuromuscular junction (NMJ) of larval <i>Drosophila</i> is widely used as a genetic model for basic neuroscience research. The presynaptic side of the NMJ is formed by axon terminals of motor neurons, the soma of which reside in the ventral ganglion of the central nervous system (CNS). Here we describe a streamlined protocol for dissection and immunostaining of the <i>Drosophila</i> CNS and NMJ that allows processing of multiple genotypes within a single staining tube. We also present a com ...[more]