Unknown

Dataset Information

0

Clinical value of microRNA-198-5p downregulation in lung adenocarcinoma and its potential pathways.


ABSTRACT: Lung adenocarcinoma (LUAD), the main subtype of non-small cell lung cancer, is known to be regulated by various microRNAs (miRs/miRNAs); however, the role of miR-198-5p in LUAD has not been clarified. In the present study, the clinical value of miR-198-5p in LUAD and its potential molecular mechanism was evaluated. miR-198-5p expression was examined by reverse transcription-quantitative PCR (RT-qPCR) in 101 paired LUAD and adjacent normal lung tissues. Subsequently, the miR-198-5p expression level was determined from microarray data from the Gene Expression Omnibus, ArrayExpress and by meta-analyses. Furthermore, the target mRNAs of miR-198-5p from 12 miRNA-mRNA predictive tools were intersected with The Cancer Genome Atlas (TCGA)-based differentially expressed genes. In addition, Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted to determine the possible mechanism of miR-198-5p in LUAD. The Search Tool for the Retrieval of Interacting Genes/Proteins database was employed to construct a protein-protein interaction network among the potential target genes of miR-198-5p. The results showed that miR-198-5p expression was lower in LUAD tissues than in adjacent non-cancerous lung tissues (4.469±2.495 vs. 5.301±2.502; P=0.015). Meta-analyses, including the data from the present study and online microarray data, also verified the downregulation of miR-198-5p in 584 cases of LUAD. The expression of miR-198-5p was associated with the age, blood vessel invasion, Tumor-Node-Metastasis stage, and lymph node metastasis of patients with LUAD and served as an independent prognostic factor for survival. The hub genes of miR-198-5p were upregulated in LUAD, according to TCGA and The Human Protein Atlas. For the KEGG pathway analysis, the most enriched KEGG pathway was the p53 signaling pathway (P=1.42×10-6). These findings indicated that the downregulation of miR-198-5p may play a pivotal role in the development of LUAD by targeting various signaling pathways.

SUBMITTER: Wang SS 

PROVIDER: S-EPMC6676716 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Clinical value of microRNA-198-5p downregulation in lung adenocarcinoma and its potential pathways.

Wang Shi-Shuo SS   Fang Ye-Ying YY   Huang Jia-Cheng JC   Liang Yue-Ya YY   Guo Yi-Nan YN   Pan Lin-Jiang LJ   Chen Gang G  

Oncology letters 20190712 3


Lung adenocarcinoma (LUAD), the main subtype of non-small cell lung cancer, is known to be regulated by various microRNAs (miRs/miRNAs); however, the role of miR-198-5p in LUAD has not been clarified. In the present study, the clinical value of miR-198-5p in LUAD and its potential molecular mechanism was evaluated. miR-198-5p expression was examined by reverse transcription-quantitative PCR (RT-qPCR) in 101 paired LUAD and adjacent normal lung tissues. Subsequently, the miR-198-5p expression lev  ...[more]

Similar Datasets

| S-EPMC5797354 | biostudies-literature
| S-EPMC7262882 | biostudies-literature
| S-EPMC8649614 | biostudies-literature
2023-03-09 | GSE146689 | GEO
| S-EPMC8157440 | biostudies-literature
| S-EPMC9117628 | biostudies-literature
| S-EPMC8312465 | biostudies-literature
| S-EPMC8351668 | biostudies-literature
| S-EPMC9161986 | biostudies-literature
| S-EPMC7099762 | biostudies-literature