Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains.
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ABSTRACT:
SUBMITTER: Raju DN
PROVIDER: S-EPMC6679001 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Publications
Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains.
Raju Diana N DN Hansen Jan N JN Rassmann Sebastian S Stüven Birthe B Jikeli Jan F JF Strünker Timo T Körschen Heinz G HG Möglich Andreas A Wachten Dagmar D
Cells 20190627 7
Inside the female genital tract, mammalian sperm undergo a maturation process called capacitation, which primes the sperm to navigate across the oviduct and fertilize the egg. Sperm capacitation and motility are controlled by 3',5'-cyclic adenosine monophosphate (cAMP). Here, we show that optogenetics, the control of cellular signaling by genetically encoded light-activated proteins, allows to manipulate cAMP dynamics in sperm flagella and, thereby, sperm capacitation and motility by light. To t ...[more]
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