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Enhanced Tolerance against a Fungal Pathogen and Insect Resistance in Transgenic Tobacco Plants Overexpressing an Endochitinase Gene from Serratia marcescens.

ABSTRACT: In this study we cloned a chitinase gene (SmchiC), from Serratia marcescens isolated from the corpse of a Diatraea magnifactella lepidopteran, which is an important sugarcane pest. The chitinase gene SmchiC amplified from the S. marcescens genome was cloned into the transformation vector p2X35SChiC and used to transform tobacco (Nicotiana tabacum L. cv Petit Havana SR1). The resistance of these transgenic plants to the necrotrophic fungus Botrytis cinerea and to the pest Spodoptera frugiperda was evaluated: both the activity of chitinase as well as the resistance against B. cinerea and S. frugiperda was significantly higher in transgenic plants compared to the wild-type.

SUBMITTER: Navarro-Gonzalez SS 

PROVIDER: S-EPMC6679225 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Enhanced Tolerance against a Fungal Pathogen and Insect Resistance in Transgenic Tobacco Plants Overexpressing an Endochitinase Gene from <i>Serratia marcescens</i>.

Navarro-González Samantha Sarai SS   Ramírez-Trujillo José Augusto JA   Peña-Chora Guadalupe G   Gaytán Paul P   Roldán-Salgado Abigail A   Corzo Gerardo G   Lina-García Laura Patricia LP   Hernández-Velázquez Víctor Manuel VM   Suárez-Rodríguez Ramón R  

International journal of molecular sciences 20190716 14

In this study we cloned a chitinase gene (<i>SmchiC</i>), from <i>Serratia marcescens</i> isolated from the corpse of a <i>Diatraea magnifactella</i> lepidopteran, which is an important sugarcane pest. The chitinase gene <i>SmchiC</i> amplified from the <i>S. marcescens</i> genome was cloned into the transformation vector p2X35SChiC and used to transform tobacco (<i>Nicotiana tabacum</i> L. cv Petit Havana SR1). The resistance of these transgenic plants to the necrotrophic fungus <i>Botrytis cin  ...[more]

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