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Rapid Characterization of Triterpene Saponins from Zornia brasiliensis by HPLC-ESI-MS/MS.

ABSTRACT: Zornia brasiliensis Vogel (Leguminosae) is a species popularly known in Brazil as "urinária", "urinana", and "carrapicho", it is popularly used as a diuretic and in the treatment of venereal diseases. A specific methodology to obtain a saponin-enriched fraction and high-performance liquid chromatography coupled with diode array detection, ion trap mass spectrometry, and TOF-MS (HPLC-DAD-ESI-MS/MS) was applied for the analysis of triterpene saponins. The MS and MS/MS experiments were carried out by ionization in negative mode. Molecular mass and fragmentation data were used to support the structural characterization of the saponins. Based on retention times, high-resolution mass determination and fragmentation, 35 oleanane-triterpene saponins were tentatively identified in Z. brasiliensis.

SUBMITTER: Nascimento YM

PROVIDER: S-EPMC6680687 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Rapid Characterization of Triterpene Saponins from Zornia brasiliensis by HPLC-ESI-MS/MS.

Nascimento Yuri Mangueira YM Abreu Lucas Silva LS Lima Ramon Leal RL Costa Vicente Carlos O VCO Melo José Iranildo Miranda de JIM Braz-Filho Raimundo R Silva Marcelo Sobral MS Tavares Josean Fechine JF

Molecules (Basel, Switzerland) 20190710 14

Zornia brasiliensis Vogel (Leguminosae) is a species popularly known in Brazil as "urinária", "urinana", and "carrapicho", it is popularly used as a diuretic and in the treatment of venereal diseases. A specific methodology to obtain a saponin-enriched fraction and high-performance liquid chromatography coupled with diode array detection, ion trap mass spectrometry, and TOF-MS (HPLC-DAD-ESI-MS/MS) was applied for the analysis of triterpene saponins. The MS and MS/MS experiments were carri ...[more]

PMID: 31295814

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