GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm.

ABSTRACT:

Background

Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination (AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments, which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm (i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential whe

SUBMITTER: Llavanera M 

PROVIDER: S-EPMC6681495 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature