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ABSTRACT: Background
This study aims to investigate the effects of CCAAT/enhancer binding protein alpha (C/EBP?) overexpression on cell proliferation, apoptosis and surfactant protein-C(SP-C) in alveolar epithelial type II (AEC II) cells after exposure to hyperoxia.Methods
pcDNA3.1(+)-C/EBP? plasmid or air-empty vector were transfected into AEC II cells with or without hyperoxia. AEC II cells were divided into air group, air+pcDNA3.1-C/EBP? group, air-empty vector group, hyperoxia group, hyperoxia+pcDNA3.1-C/EBP? group, and hyperoxia-empty vector group. Cell proliferation was analyzed using Cell Counting Kit-8. The mRNA level and protein expression were measured using PCR and Western blot techniques, respectively. The cell cycle and apoptosis were analyzed using flow cytometry.Results
After 48?h of post-transfection, significantly higher protein expression of C/EBP? was observed in the C/EBP? transfection group with or without hyperoxia compared to the others (P?ConclusionC/EBP? overexpression significantly upregulates the expression of SP-C, promotes cell proliferation, and inhibits apoptosis in AEC II cells after exposure to hyperoxia. Hence, this data suggests that C/EBP? overexpression may reverse the damage and exert a protective role in hyperoxia-induced lung injury.
SUBMITTER: Lu H
PROVIDER: S-EPMC6683353 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Lu Hongyan H Chen Xiaoqing X Lu Yanmin Y Zhu Haitao H Tang Wei W Wang Qiuxia Q
BMC pulmonary medicine 20190806 1
<h4>Background</h4>This study aims to investigate the effects of CCAAT/enhancer binding protein alpha (C/EBPα) overexpression on cell proliferation, apoptosis and surfactant protein-C(SP-C) in alveolar epithelial type II (AEC II) cells after exposure to hyperoxia.<h4>Methods</h4>pcDNA3.1(+)-C/EBPα plasmid or air-empty vector were transfected into AEC II cells with or without hyperoxia. AEC II cells were divided into air group, air+pcDNA3.1-C/EBPα group, air-empty vector group, hyperoxia group, h ...[more]