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Mass spectrometry imaging as a tool for evaluating the pulmonary distribution of exogenous surfactant in premature lambs.


ABSTRACT:

Background

The amount of surfactant deposited in the lungs and its overall pulmonary distribution determine the therapeutic outcome of surfactant replacement therapy. Most of the currently available methods to determine the intrapulmonary distribution of surfactant are time-consuming and require surfactant labelling. Our aim was to assess the potential of Mass Spectrometry Imaging (MSI) as a label-free technique to qualitatively and quantitatively evaluate the distribution of surfactant to the premature lamb.

Methods

Twelve preterm lambs (gestational age 126-127d, term ~150d) were allocated in two experimental groups. Seven lambs were treated with an intratracheal bolus of the synthetic surfactant CHF5633 (200?mg/kg) and 5 lambs were managed with mechanical ventilation for 120?min, as controls. The right lung lobes of all lambs were gradually frozen while inflated to 20 cmH2O pressure for lung cryo-sections for MSI analysis. The intensity signals of SP-C analog and SP-B analog, the two synthetic peptides contained in the CHF5633 surfactant, were used to locate, map and quantify the intrapulmonary exogenous surfactant.

Results

Surfactant treatment was associated with a significant improvement of the mean arterial oxygenation and lung compliance (p?ConclusionsApplying MSI, we were able to detect, locate and quantify the amount of exogenous surfactant distributed to the lower right lung of surfactant-treated lambs. The distribution pattern of SP-B analog correlated well with the pulmonary physiological outcomes of the animals. MSI is a valuable label-free technique which is able to simultaneously evaluate qualitative and quantitative drug distribution in the lung.

SUBMITTER: Zecchi R 

PROVIDER: S-EPMC6683365 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Mass spectrometry imaging as a tool for evaluating the pulmonary distribution of exogenous surfactant in premature lambs.

Zecchi Riccardo R   Franceschi Pietro P   Tigli Laura L   Ricci Francesca F   Boscaro Francesca F   Pioselli Barbara B   Mileo Valentina V   Murgia Xabier X   Bianco Federico F   Salomone Fabrizio F   Schmidt Augusto F AF   Hillman Noah H NH   Kemp Matthew W MW   Jobe Alan H AH  

Respiratory research 20190805 1


<h4>Background</h4>The amount of surfactant deposited in the lungs and its overall pulmonary distribution determine the therapeutic outcome of surfactant replacement therapy. Most of the currently available methods to determine the intrapulmonary distribution of surfactant are time-consuming and require surfactant labelling. Our aim was to assess the potential of Mass Spectrometry Imaging (MSI) as a label-free technique to qualitatively and quantitatively evaluate the distribution of surfactant  ...[more]

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