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Increased circulating Tfh to Tfr ratio in chronic renal allograft dysfunction: a pilot study.

ABSTRACT: BACKGROUND:T follicular helper (Tfh) cells play a control role in contribution of B cell differentiation and antibody production. T follicular regulatory (Tfr) cells inhibit Tfh-B cell interaction. METHODS:To identify whether circulating Tfh (cTfh) and Tfr (cTfr) cells contribute to chronic renal allograft dysfunction (CAD), 67 kidney transplant recipients (34 recipients with CAD, 33 recipients with stable function) were enrolled. The frequency of cTfh and cTfr cells, the level of serum CXCL13 were measured. RESULTS:The frequency of cTfr cells in CAD group was significantly lower than that in stable group (0.31% vs 0.68%, P?=?0.002). The cTfh to cTfr ratio in CAD group was significantly higher than that in stable group (55.4 vs 25.3, P?=?0.013). Serum CXCL13 in CAD group was significantly higher than stable group (30.4 vs 21.9?ng/ml, P?=?0.025). After linear regression analysis, the cTfh to cTfr ratio was an independent risk factor for estimated glomerular filtration rate (eGFR) in recipients (standardized coefficient?=?-?0.420, P?=?0.012). After logistic regression analysis, the cTfh to cTfr ratio was an independent risk factor for CAD (OR?=?1.043, 95%CI?=?1.004-1.085, P?=?0.031). CONCLUSION:The imbalance between cTfh and cTfr cells contribute to the development of CAD.

SUBMITTER: Yan L

PROVIDER: S-EPMC6683539 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Increased circulating Tfh to Tfr ratio in chronic renal allograft dysfunction: a pilot study.

Yan Lin L Li Yamei Y Li Yi Y Wu Xiaojuan X Wang Xianding X Wang Lanlan L Shi Yunying Y Tang Jiangtao J

BMC immunology 20190805 1

<h4>Background</h4>T follicular helper (Tfh) cells play a control role in contribution of B cell differentiation and antibody production. T follicular regulatory (Tfr) cells inhibit Tfh-B cell interaction.<h4>Methods</h4>To identify whether circulating Tfh (cTfh) and Tfr (cTfr) cells contribute to chronic renal allograft dysfunction (CAD), 67 kidney transplant recipients (34 recipients with CAD, 33 recipients with stable function) were enrolled. The frequency of cTfh and cTfr cells, the level of ...[more]

PMID: 31382877

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Project description:Background: Follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells are essential for B cell differentiation, germinal center formation, and humoral immune responses. Immunity and inflammation have been thought to be involved in Parkinson's disease (PD). In this study, we aimed to identify whether circulating Tfh and Tfr (cTfh and cTfr) cells contribute to PD. Methods: Thirty-nine PD patients and 26 health controls (HCs) were enrolled. The numbers of cTfh (CD4+CXCR5+PD-1+) cells and cTfr (CD4+CXCR5+CD25hiCD127low) cells were analyzed via flow cytometry. The serum concentrations of interleukin (IL)-4, IL-10, IL-21, and transforming growth factor (TGF)-β were examined by cytometric bead array. Results: The percentage of cTfh cells among CD4+ T cells in PD patients was significantly higher than that in HCs [3.68% (2.64-5.70%) vs. 1.94% (1.32%-2.99%), P < 0.001], while the percentage of cTfr cells among CD4+ T cells in PD patients was slight decreased but without significance [1.05% (0.62-1.54%) vs. 1.3% (0.63-1.90%), P > 0.05]. The percentage of CD19+ B cells in peripheral blood mononuclear cells was significantly lower in PD patients than in HCs [5.35% (4.13-9.38%) vs. 8.68% (5.61-12.93%), P = 0.014]. The serum concentrations of IL-4, IL-10, IL-21, and TGF-β in PD patients did not differ significantly from those in HCs (P > 0.05). There was a positive trend of the correlation between the number of cTfh and the serum IL-4 concentrations in PD patients (P = 0.032, r = 0.353). There was a positive trend of the correlation between the number of cTfr and the serum IL-10 concentrations in PD patients (P = 0.047, r = 0.328), A positive trend of the correlation were found for the serum concentration of IL-21 with H-Y stage (r = 0.356, P = 0.026) and UPDRS-III score (r = 0.347, P = 0.030). Conclusions: These results indicate that an imbalance of cTfh and cTfr cells may be involved in the chronic progression of PD, and IL-21 may be a biomarker for monitoring the severity of this disease.

Dataset Information

Increased circulating Tfh to Tfr ratio in chronic renal allograft dysfunction: a pilot study.

Publications

Increased circulating Tfh to Tfr ratio in chronic renal allograft dysfunction: a pilot study.

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