Dataset Information

IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8?+ Dendritic Cells Promotes Listeria monocytogenes Infection.

ABSTRACT: Type 1 CD8?+ conventional dendritic cells (cDC1s) are required for CD8+ T cell priming but, paradoxically, promote splenic Listeria monocytogenes infection. Using mice with impaired cDC2 function, we ruled out a role for cDC2s in this process and instead discovered an interleukin-10 (IL-10)-dependent cellular crosstalk in the marginal zone (MZ) that promoted bacterial infection. Mice lacking the guanine nucleotide exchange factor DOCK8 or CD19 lost IL-10-producing MZ B cells and were resistant to Listeria. IL-10 increased intracellular Listeria in cDC1s indirectly by reducing inducible nitric oxide synthase expression early after infection and increasing intracellular Listeria in MZ metallophilic macrophages (MMMs). These MMMs trans-infected cDC1s, which, in turn, transported Listeria into the white pulp to prime CD8+ T cells. However, this also facilitated bacterial expansion. Therefore, IL-10-mediated crosstalk between B cells, macrophages, and cDC1s in the MZ promotes both Listeria infection and CD8+ T cell activation.

SUBMITTER: Liu D

PROVIDER: S-EPMC6685086 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α+ Dendritic Cells Promotes Listeria monocytogenes Infection.

Liu Dong D Yin Xiangyun X Olyha Sam J SJ Nascimento Manuela Sales L MSL Chen Pei P White Theresa T Gowthaman Uthaman U Zhang Tingting T Gertie Jake A JA Zhang Biyan B Xu Lan L Yurieva Marina M Devine Lesley L Williams Adam A Eisenbarth Stephanie C SC

Immunity 20190620 1

Type 1 CD8α+ conventional dendritic cells (cDC1s) are required for CD8+ T cell priming but, paradoxically, promote splenic Listeria monocytogenes infection. Using mice with impaired cDC2 function, we ruled out a role for cDC2s in this process and instead discovered an interleukin-10 (IL-10)-dependent cellular crosstalk in the marginal zone (MZ) that promoted bacterial infection. Mice lacking the guanine nucleotide exchange factor DOCK8 or CD19 lost IL-10-producing MZ B cell ...[more]

PMID: 31231033

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Dataset Information

IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8?+ Dendritic Cells Promotes Listeria monocytogenes Infection.

Publications

IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α<sup>+</sup> Dendritic Cells Promotes Listeria monocytogenes Infection.

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