Ontology highlight
ABSTRACT:
SUBMITTER: Klimkowski P
PROVIDER: S-EPMC6686645 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Klimkowski Piotr P De Ornellas Sara S Singleton Daniel D El-Sagheer Afaf H AH Brown Tom T
Organic & biomolecular chemistry 20190603 24
We have synthesised a range of thiazole orange (TO) functionalised oligonucleotides for nucleic acid detection in which TO is attached to the nucleobase or sugar of thymidine. The properties of duplexes between TO-probes and their DNA and RNA targets strongly depend on the length of the linker between TO and the oligonucleotide, the position of attachment of TO to the nucleotide (major or minor groove) and the mode of attachment of thiazole orange (via benzothiazole or quinoline moiety). This in ...[more]