Dataset Information

HIV-1 is rarely detected in blood and colon myeloid cells during viral-suppressive antiretroviral therapy.

ABSTRACT:

Objective

The aim of this study was to explore the contribution of blood and colon myeloid cells to HIV persistence during antiretroviral therapy (ART).

Design

Leukapheresis was collected from HIV-infected individuals with undetectable plasma viral load during ART (HIV + ART; n = 15) and viremics untreated (HIV+; n = 6). Rectal sigmoid biopsies were collected from n = 8 HIV+ART.

Methods

Myeloid cells (total monocytes (Mo), CD16/CD16 Mo, CD1c dendritic cells) and CD4 T cells were isolated by magnetic-activated cell sorting (MACS) and/or fluorescence-activated cell sorting (FACS) from peripheral blood. Matched myeloid and CCR6CD4 T cells were isolated from blood and rectal biopsies by FACS. Levels of early (RU5 primers), late (Gag primers) and/or integrated HIV-DNA (Alu/HIV primers) were quantified by nested real-time PCR. Replication-competent HIV was amplified by co-culturing cells from HIV-positive individuals with CD3/CD28-activated CD4 T cells from uninfected donors.

Results

Early/late but not integrated HIV reverse transcripts were detected in blood myeloid subsets of four out of 10 HIV+ART; in contrast, integrated HIV-DNA was exclusively detected in CD4 T cells. In rectal biopsies, late HIV reverse transcripts were detected in myeloid cells and CCR6CD4 T cells from one out of eight and seven out of eight HIV+ART individuals, respectively. Replication-competent HIV was outgrown from CD4 T cells but not from myeloid of untreated/ART-treated HIV-positive individuals.

Conclusion

In contrast to CD4 T cells, blood and colon myeloid cells carry detectable HIV only in a small fraction of HIV+ART individuals. This is consistent with the documented resistance of Mo to HIV infection and the rapid turnover of Mo-derived macrophages in the colon. Future assessment of multiple lymphoid and nonlymphoid tissues is required to include/exclude myeloid cells as relevant HIV reservoirs during ART.

SUBMITTER: Cattin A

PROVIDER: S-EPMC6686847 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Publications

HIV-1 is rarely detected in blood and colon myeloid cells during viral-suppressive antiretroviral therapy.

Cattin Amélie A Wiche Salinas Tomas Raul TR Gosselin Annie A Planas Delphine D Shacklett Barbara B Cohen Eric A EA Ghali Maged P MP Routy Jean-Pierre JP Ancuta Petronela P

AIDS (London, England) 20190701 8

<h4>Objective</h4>The aim of this study was to explore the contribution of blood and colon myeloid cells to HIV persistence during antiretroviral therapy (ART).<h4>Design</h4>Leukapheresis was collected from HIV-infected individuals with undetectable plasma viral load during ART (HIV + ART; n = 15) and viremics untreated (HIV+; n = 6). Rectal sigmoid biopsies were collected from n = 8 HIV+ART.<h4>Methods</h4>Myeloid cells (total monocytes (Mo), CD16/CD16 Mo, CD1c dendritic cells) and CD4 T cells ...[more]

PMID: 30870200

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Dataset Information

HIV-1 is rarely detected in blood and colon myeloid cells during viral-suppressive antiretroviral therapy.

Objective

Design

Methods

Results

Conclusion

Publications

HIV-1 is rarely detected in blood and colon myeloid cells during viral-suppressive antiretroviral therapy.

Similar Datasets

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