Unknown

Dataset Information

0

Population Genome Sequencing of the Scab Fungal Species Venturia inaequalis, Venturia pirina, Venturia aucupariae and Venturia asperata.


ABSTRACT: The Venturia genus comprises fungal species that are pathogens on Rosaceae host plants, including V. inaequalis and V. asperata on apple, V. aucupariae on sorbus and V. pirina on pear. Although the genetic structure of V. inaequalis populations has been investigated in detail, genomic features underlying these subdivisions remain poorly understood. Here, we report whole genome sequencing of 87 Venturia strains that represent each species and each population within V. inaequalis We present a PacBio genome assembly for the V. inaequalis EU-B04 reference isolate. The size of selected genomes was determined by flow cytometry, and varied from 45 to 93 Mb. Genome assemblies of V. inaequalis and V. aucupariae contain a high content of transposable elements (TEs), most of which belong to the Gypsy or Copia LTR superfamilies and have been inactivated by Repeat-Induced Point mutations. The reference assembly of V. inaequalis presents a mosaic structure of GC-equilibrated regions that mainly contain predicted genes and AT-rich regions, mainly composed of TEs. Six pairs of strains were identified as clones. Single-Nucleotide Polymorphism (SNP) analysis between these clones revealed a high number of SNPs that are mostly located in AT-rich regions due to misalignments and allowed determining a false discovery rate. The availability of these genome sequences is expected to stimulate genetics and population genomics research of Venturia pathogens. Especially, it will help understanding the evolutionary history of Venturia species that are pathogenic on different hosts, a history that has probably been substantially influenced by TEs.

SUBMITTER: Le Cam B 

PROVIDER: S-EPMC6686934 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Population Genome Sequencing of the Scab Fungal Species <i>Venturia inaequalis</i>, <i>Venturia pirina</i>, <i>Venturia aucupariae</i> and <i>Venturia asperata</i>.

Le Cam Bruno B   Sargent Dan D   Gouzy Jérôme J   Amselem Joëlle J   Bellanger Marie-Noëlle MN   Bouchez Olivier O   Brown Spencer S   Caffier Valérie V   De Gracia Marie M   Debuchy Robert R   Duvaux Ludovic L   Payen Thibaut T   Sannier Mélanie M   Shiller Jason J   Collemare Jérôme J   Lemaire Christophe C  

G3 (Bethesda, Md.) 20190808 8


The <i>Venturia</i> genus comprises fungal species that are pathogens on <i>Rosaceae</i> host plants, including <i>V. inaequalis</i> and <i>V. asperata</i> on apple, <i>V. aucupariae</i> on sorbus and <i>V. pirina</i> on pear. Although the genetic structure of <i>V. inaequalis</i> populations has been investigated in detail, genomic features underlying these subdivisions remain poorly understood. Here, we report whole genome sequencing of 87 <i>Venturia</i> strains that represent each species an  ...[more]

Similar Datasets

| S-EPMC6256676 | biostudies-literature
| S-EPMC5025049 | biostudies-literature
| S-EPMC3547962 | biostudies-literature
| S-EPMC6834240 | biostudies-literature
| S-EPMC5775622 | biostudies-literature
2022-03-14 | GSE198244 | GEO
| S-EPMC4646964 | biostudies-literature
| S-EPMC7825683 | biostudies-literature
| S-EPMC11374964 | biostudies-literature
| S-EPMC8191898 | biostudies-literature