Dataset Information
Simultaneous quantification of lidocaine and prilocaine in human plasma by LC-MS/MS and its application in a human pharmacokinetic study.
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ABSTRACT: Objective
The aim of the work was to develop and validate a simple, sensitive and selective Liquid chromatography with Mass spectroscopic method for simultaneous quantification of lidocaine and prilocaine in human plasma.Design and methods
Analytes and the internal standards from human plasma were extracted by using solid- phase extraction technique using Waters Oasis® HLB 1 ?cc (30 ?mg) cartridges. The reconstituted samples were chromatographed on Phenomenex Kinetex EVO 4.6*100 ?mm 2.6 ? 100A column by using a mixture of acetonitrile and 5 ?mM ammonium acetate buffer (80:20, v/v) as the mobile phase at a flow rate of 0.6 ?mL/min.Results
The method was validated over the concentration range of 0.10-201.80?ng/mL for lidocaine and 0.10-201.66?ng/mL for prilocaine. The calibration curve obtained was linear.Conclusion
Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 3.0?min for each sample, make it possible to analyze more than 350 human plasma samples per day. The proposed method was found applicable for pharmacokinetic studies.
SUBMITTER: Rao Yadlapalli SS
PROVIDER: S-EPMC6687230 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
Simultaneous quantification of lidocaine and prilocaine in human plasma by LC-MS/MS and its application in a human pharmacokinetic study.
Practical laboratory medicine 20190730
<h4>Objective</h4>The aim of the work was to develop and validate a simple, sensitive and selective Liquid chromatography with Mass spectroscopic method for simultaneous quantification of lidocaine and prilocaine in human plasma.<h4>Design and methods</h4>Analytes and the internal standards from human plasma were extracted by using solid- phase extraction technique using Waters Oasis® HLB 1 cc (30 mg) cartridges. The reconstituted samples were chromatographed on Phenomenex Kinetex EVO 4.6*100 ...[more]