Dataset Information

Identification, Expression Patterns and RNA Interference of Aquaporins in Dendroctonus armandi (Coleoptera: Scolytinae) Larvae During Overwintering.

ABSTRACT: The ability to survive annual temperature minima could be a key determinant of distribution limits for insects under global climate change. Recent studies have suggested that insect aquaporins are indispensable for cellular water management under conditions that lead to dehydration and cold stress. Aquaporins are integral membrane water channel proteins in the major intrinsic protein superfamily and promote selected solutes and the movement of water across biological membranes. We cloned and characterized nine full-length aquaporins from Dendroctonus armandi (DaAqps), the most destructive forest pest in the Qinling Mountains of Shaanxi Province, China. Eight of the DaAqps belong to three classical aquaporin grades, including the Drosophila integral protein, the Pyrocoelia rufa integral protein, the entomoglyceroporins and one that belongs to the unorthodox grade of aquaporin 12-like channels. The DaAqps were increasingly expressed during different developmental stages and in different larval tissues, and expression peaked in mid-winter. They were tested under cold conditions for different lengths of time, and the expression of almost all DaAqps was down regulated with decreasing temperatures and long-term exposure to cold conditions. However, when the lowest temperatures were reached, the levels were immediately upregulated. These genes indicate that cold tolerance can improve through mortality responses at low temperatures after RNA interference of DaAqps. In our study, we analyzed the molecular response, expression patterns, and RNA interference of DaAqps and clarified the crucial role of protective compounds (aquaporins) underlying D. armandi cold tolerance and provide a new pest control method.

SUBMITTER: Fu D

PROVIDER: S-EPMC6688586 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Identification, Expression Patterns and RNA Interference of Aquaporins in <i>Dendroctonus armandi</i> (Coleoptera: Scolytinae) Larvae During Overwintering.

Fu Danyang D Dai Lulu L Gao Haiming H Sun Yaya Y Liu Bin B Chen Hui H

Frontiers in physiology 20190802

The ability to survive annual temperature minima could be a key determinant of distribution limits for insects under global climate change. Recent studies have suggested that insect aquaporins are indispensable for cellular water management under conditions that lead to dehydration and cold stress. Aquaporins are integral membrane water channel proteins in the major intrinsic protein superfamily and promote selected solutes and the movement of water across biological membranes. We cloned and cha ...[more]

PMID: 31427984

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Project description:BackgroundThe European spruce bark beetle, Ips typographus, and the North American mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae: Scolytinae), are severe pests of coniferous forests. Both bark beetle species utilize aggregation pheromones to coordinate mass-attacks on host trees, while odorants from host and non-host trees modulate the pheromone response. Thus, the bark beetle olfactory sense is of utmost importance for fitness. However, information on the genes underlying olfactory detection has been lacking in bark beetles and is limited in Coleoptera. We assembled antennal transcriptomes from next-generation sequencing of I. typographus and D. ponderosae to identify members of the major chemosensory multi-gene families.ResultsGene ontology (GO) annotation indicated that the relative abundance of transcripts associated with specific GO terms was highly similar in the two species. Transcripts with terms related to olfactory function were found in both species. Focusing on the chemosensory gene families, we identified 15 putative odorant binding proteins (OBP), 6 chemosensory proteins (CSP), 3 sensory neuron membrane proteins (SNMP), 43 odorant receptors (OR), 6 gustatory receptors (GR), and 7 ionotropic receptors (IR) in I. typographus; and 31 putative OBPs, 11 CSPs, 3 SNMPs, 49 ORs, 2 GRs, and 15 IRs in D. ponderosae. Predicted protein sequences were compared with counterparts in the flour beetle, Tribolium castaneum, the cerambycid beetle, Megacyllene caryae, and the fruit fly, Drosophila melanogaster. The most notable result was found among the ORs, for which large bark beetle-specific expansions were found. However, some clades contained receptors from all four beetle species, indicating a degree of conservation among some coleopteran OR lineages. Putative GRs for carbon dioxide and orthologues for the conserved antennal IRs were included in the identified receptor sets.ConclusionsThe protein families important for chemoreception have now been identified in three coleopteran species (four species for the ORs). Thus, this study allows for improved evolutionary analyses of coleopteran olfaction. Identification of these proteins in two of the most destructive forest pests, sharing many semiochemicals, is especially important as they might represent novel targets for population control.

Dataset Information

Identification, Expression Patterns and RNA Interference of Aquaporins in Dendroctonus armandi (Coleoptera: Scolytinae) Larvae During Overwintering.

Publications

Identification, Expression Patterns and RNA Interference of Aquaporins in <i>Dendroctonus armandi</i> (Coleoptera: Scolytinae) Larvae During Overwintering.

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