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Molecular cloning and functional analysis of a ?12-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L.

ABSTRACT: Chlamydomonas sp. ICE-L, which can thrive in extreme environments of the Antarctic, could represent a promising alternative for polyunsaturated fatty acid (PUFA) production. A new ?12-fatty acid desaturase (FAD)-encoding gene (? 12 CiFAD), 1269 bp in size, was cloned from Chlamydomonas sp. ICE-L. Bioinformatics analysis showed that ? 12 CiFAD-encoded protein was homologous to known FADs with conserved histidine motifs, and localized to the chloroplast. Functional analysis of ? 12 CiFAD indicated that recombinant Synechococcus 6803 expressing ?12CiFAD could accumulate C18:2, whereas recombinant Saccharomyces cerevisiae expressing this enzyme could not accumulate C18:2 or any other new fatty acids. These results indicate that ?12CiFAD is a functional enzyme in the chloroplast that can adjust Chlamydomonas sp. ICE-L cell membrane fluidity to adapt to Antarctic extreme low-temperature environments, which give us insights into the frigostable and cold-resistant mechanisms of hypothermic organisms.

SUBMITTER: He Y 

PROVIDER: S-EPMC6689313 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Molecular cloning and functional analysis of a Δ<sup>12</sup>-fatty acid desaturase from the Antarctic microalga <i>Chlamydomonas</i> sp. ICE-L.

He Yingying Y   Zheng Zhou Z   An Meiling M   Chen Hao H   Qu Changfeng C   Liu Fangming F   Wang Yibin Y   Miao Jinlai J   Hou Xuguang X  

3 Biotech 20190810 9

<i>Chlamydomonas</i> sp. ICE-L, which can thrive in extreme environments of the Antarctic, could represent a promising alternative for polyunsaturated fatty acid (PUFA) production. A new Δ<sup>12</sup>-fatty acid desaturase (FAD)-encoding gene (<i>Δ</i> <sup><i>12</i></sup> <i>CiFAD</i>), 1269 bp in size, was cloned from <i>Chlamydomonas</i> sp. ICE-L. Bioinformatics analysis showed that <i>Δ</i> <sup><i>12</i></sup> <i>CiFAD</i>-encoded protein was homologous to known FADs with conserved histid  ...[more]

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