Ontology highlight
ABSTRACT:
SUBMITTER: Wright CM
PROVIDER: S-EPMC6690184 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Wright Christine M CM Whitaker Robert H RH Onuiri Joshua E JE Blackburn Tessa T McGarity Sierra S Bjornsti Mary-Ann MA Placzek William J WJ
Biochemistry 20190110 6
SUMO, a conserved ubiquitin-like protein, is conjugated to a multitude of cellular proteins to maintain genomic integrity and resist genotoxic stress. Studies of the SUMO E2 conjugating enzyme mutant, UBC9<sub>P123L</sub>, suggested that altered substrate specificity enhances cell sensitivity to DNA damaging agents. Using nuclear magnetic resonance chemical shift studies, we confirm that the mutation does not alter the core globular fold of UBC9, while <sup>15</sup>N relaxation measurements demo ...[more]