Ontology highlight
ABSTRACT:
SUBMITTER: Liang M
PROVIDER: S-EPMC6694116 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Liang Mindong M Li Zilong Z Wang Weishan W Liu Jiakun J Liu Leshi L Zhu Guoliang G Karthik Loganathan L Wang Man M Wang Ke-Feng KF Wang Zhong Z Yu Jing J Shuai Yuting Y Yu Jiaming J Zhang Lu L Yang Zhiheng Z Li Chuan C Zhang Qian Q Shi Tong T Zhou Liming L Xie Feng F Dai Huanqin H Liu Xueting X Zhang Jingyu J Liu Guang G Zhuo Ying Y Zhang Buchang B Liu Chenli C Li Shanshan S Xia Xuekui X Tong Yaojun Y Liu Yanwen Y Alterovitz Gil G Tan Gao-Yi GY Zhang Li-Xin LX
Nature communications 20190814 1
Besides genome editing, CRISPR-Cas12a has recently been used for DNA detection applications with attomolar sensitivity but, to our knowledge, it has not been used for the detection of small molecules. Bacterial allosteric transcription factors (aTFs) have evolved to sense and respond sensitively to a variety of small molecules to benefit bacterial survival. By combining the single-stranded DNA cleavage ability of CRISPR-Cas12a and the competitive binding activities of aTFs for small molecules an ...[more]