Comparable type I interferon score determination from PAXgene and Tempus whole blood RNA collection and isolation systems.

ABSTRACT:

Objective

Type I interferons (IFN) have important roles in many immune-mediated inflammatory diseases (IMIDs) and are a relatively new therapeutic target. Direct detection of type I IFNs has proved challenging, thus their presence is often inferred from the expression of interferon-stimulated genes (ISGs) and calculation of an interferon score (IS). The objective of this research was to determine if the expression of six common ISGs and subsequent IS were comparable when RNA was derived from the Tempus and PAXgene whole blood RNA collection systems.

Results

Whole blood was obtained from ten healthy adults, incubated ex vivo in the absence and presence of recombinant human IFNα then divided into PAXgene and Tempus tubes. Despite reports of tube-specific patterns of gene expre

SUBMITTER: Lamot L 

PROVIDER: S-EPMC6694656 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature