Citrulline Effect Is a Characteristic Feature of Deiminated Peptides in Tandem Mass Spectrometry.

ABSTRACT: Tandem mass spectrometry of peptides is of utmost importance in proteomics. Collision-induced dissociation usually generates y type fragment ion series from tryptic peptides, carrying information on their primary structure. Amino acid side chains or differences in their basicity could alter fragmentation processes considerably. The well-known proline effect is a cleavage preference at the N-terminus of proline residues in peptides, usually yielding a very abundant y ion while suppressing others. Previously, we reported a similar phenomenon occurring at the C-terminus of citrulline residues and coined the term Cit effect. To confirm the presence of Cit effect in large proteomic datasets, we analyzed 293 peptides containing Cit residues based on the human proteome database mining work of Lee

SUBMITTER: Steckel A 

PROVIDER: S-EPMC6695478 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature