Dataset Information

Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco.

ABSTRACT: Cadmium (Cd) is an environmental pollutant that potentially threatens human health worldwide. Developing approaches for efficiently treating environmental Cd is a priority. Selenium (Se) plays important role in the protection of plants against various abiotic stresses, including heavy metals. Previous research has shown that Se can alleviate Cd toxicity, but the molecular mechanism is still not clear. In this study, we explore the function of auxin and phosphate (P) in tobacco (Nicotiana tabacum), with particular focus on their interaction with Se and Cd. Under Cd stress conditions, low Se (10 ?M) significantly increased the biomass and antioxidant capacity of tobacco plants and reduced uptake of Cd. We also measured the auxin concentration and expression of auxin-relative genes in tobacco and found that plants treated with low Se (10 ?M) had higher auxin concentrations at different Cd supply levels (0 ?M, 20 ?M, 50 ?M) compared with no Se treatment, probably due to increased expression of auxin synthesis genes and auxin efflux carriers. Overexpression of a high affinity phosphate transporter NtPT2 enhanced the tolerance of tobacco to Cd stress, possibly by increasing the total P and Se content and decreasing Cd accumulation compared to that in the wild type (WT). Our results show that there is an interactive mechanism among P, Se, Cd, and auxin that affects plant growth and may provide a new approach for relieving Cd toxicity in plants.

SUBMITTER: Luo Y

PROVIDER: S-EPMC6696094 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco.

Luo Yong Y Wei Yuewei Y Sun Shuguang S Wang Jian J Wang Weifeng W Han Dan D Shao Huifang H Jia Hongfang H Fu Yunpeng Y

International journal of molecular sciences 20190801 15

Cadmium (Cd) is an environmental pollutant that potentially threatens human health worldwide. Developing approaches for efficiently treating environmental Cd is a priority. Selenium (Se) plays important role in the protection of plants against various abiotic stresses, including heavy metals. Previous research has shown that Se can alleviate Cd toxicity, but the molecular mechanism is still not clear. In this study, we explore the function of auxin and phosphate (P) in tobacco (<i>Nicotiana taba ...[more]

PMID: 31374993

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Project description:Intensive insight into the potential mechanisms of Se-induced Cd tolerance in cucumber seedlings is essential for further improvement of vegetable crop cultivation and breeding to obtain high yields and quality in Cd-contaminated soil. To reveal the ultrastructural and metabolic differences in Se-induced Cd tolerance, we examined the ultrastructures of chloroplasts and root cells and characterised 155 differentially expressed metabolites under Cd and/or Se stress using gas chromatography-mass spectrometry (GC-MS)-based metabolomics. Exogenous Se greatly relieved Cd-caused injuries to the ultrastructures of cucumber leaves and roots; for example, the shapes of chloroplasts treated with Cd + Se improved or even began to return to normal, the nuclei of root cells began to regenerate better and the chromatin was well-distributed compared with plants treated with Cd alone. Metabolite profiling revealed several intermediates of glycolysis and the tricarboxylic acid (TCA) cycle; also, some amino acids were up-accumulated in Cd + Se-treated cucumber seedlings and down-accumulated in Cd-treated cucumber seedlings, such as pyruvic acid, galactose, lactose, glutaric acid and alanine in leaves, glucose-6-phosphate and serine in roots, and lactic acid and glycine in both leaves and roots. These metabolites may play dominant roles in developing Se-mediated Cd tolerance. Moreover, a high level of sugars and polyols, amino acids and organic acids were up-accumulated in Cd-treated plants. Meanwhile, our data suggest that high accumulation of fructose, α-ketoglutaric acid, shikimic acid, fumaric acid and succinic acid in roots is a Cd-specific response, indicating that these metabolites are vital for cucumbers to develop Cd resistance. This study extends the current understanding of the mechanisms of Se in abating Cd contamination in cucumber and demonstrates that metabolomics profiling provides a more comprehensive view of the response of plants to heavy metals.

Dataset Information

Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco.

Publications

Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco.

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