Dataset Information

Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens).

ABSTRACT: Hermetia illucens is an important resource insect for the conversion of organic waste. Quantitative PCR (qPCR) is the primary tool of gene expression analysis and a core technology of molecular biology research. Reference genes are essential for qPCR analysis; however, a stability analysis of H. illucens reference genes has not yet been carried out. To find suitable reference genes for normalizing gene expression data, the stability of eight housekeeping genes (including ATP6V1A, RPL8, EF1, Tubulin, TBP, GAPDH, Actin and RP49) was investigated under both biotic (developmental stages, tissues and sex) and abiotic (heavy metals, food, antibiotics) conditions. Gene expression data were analysed by geNorm, NormFinder, BestKeeper, and ?Ct programs. A set of specific reference genes was recommended for each experimental condition using the results of RefFinder synthesis analysis. This study offers a solid foundation for further studies of the molecular biology of H. illucens.

SUBMITTER: Gao Z

PROVIDER: S-EPMC6697329 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens).

Gao Zhenghui Z Deng Wenhui W Zhu Fen F

PloS one 20190816 8

Hermetia illucens is an important resource insect for the conversion of organic waste. Quantitative PCR (qPCR) is the primary tool of gene expression analysis and a core technology of molecular biology research. Reference genes are essential for qPCR analysis; however, a stability analysis of H. illucens reference genes has not yet been carried out. To find suitable reference genes for normalizing gene expression data, the stability of eight housekeeping genes (including ATP6V1A, RPL8, EF1, Tubu ...[more]

PMID: 31419256

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Project description:The black soldier fly (BSF), Hermetia illucens (L., 1758) (Diptera: Stratiomyidae), is an endemic fly species from the tropical, subtropical and warm temperate zones of America. This saprophagous species relies on its environment where it finds the decomposing matter for the larvae to grow. The polyphagous diet and the macronutrient quality (mainly lipids and proteins) of these larvae make them excellent candidates for various applications such as waste and organic material management, incorporation in animal feed or alternative energy source. Although rearing development in temperate regions requires artificial processes to continuously produce high quality eggs and larvae, few studies have been conducted on the mating and oviposition processes governing H. illucens reproduction. Research conducted in semi-artificial rearing conditions showed that the number of mating varied according to the season. It has been speculated that this behavior could be due to differences in the intensity of sunlight caused by the change of seasons. This study aims at evaluating the influence of sex-ratio, density and nycthemeral cycle on H. illucens reproduction. In order to tackle this issue, an artificial set up for oviposition to collect eggs has been developed. This egg collection system aims at centralize oviposition and simplify eggs collection. Two populations with opposite sex-ratio (male-dominant and female-dominant) were selected. Their respective eggs productions have been evaluated for five breeding densities. Eggs weights varied significantly among the densities for each opposite sex-ratio population and female dominant population produced most eggs weight from 6500 individuals /m3. Finally, four nycthemeral cycles (2, 6, 12 and 18h of daily light) were simulated to evaluate the impact of light duration on reproduction. Early oviposition pic associated with a decrease of the oviposition period are shown when H. illucens are exposed to increasing light duration. These experiments enable improvement of the understanding on artificial reproduction of H. illucens.

Dataset Information

Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens).

Publications

Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens).

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