MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy.
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ABSTRACT: The non-destructive collection of ultrathin sections on silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling the remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.
SUBMITTER: Templier T
PROVIDER: S-EPMC6697447 | biostudies-literature |
REPOSITORIES: biostudies-literature
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