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MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy.

ABSTRACT: The non-destructive collection of ultrathin sections on silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling the remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.

SUBMITTER: Templier T

PROVIDER: S-EPMC6697447 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy.

Templier Thomas T

eLife 20190712

The non-destructive collection of ultrathin sections on silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling the remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric el ...[more]

PMID: 31294691

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