Dataset Information

L-glutamine protects mouse brain from ischemic injury via up-regulating heat shock protein 70.

ABSTRACT:

Introduction

L-glutamine is an antioxidant that plays a role in a variety of biochemical processes. Given that oxidative stress is a key component of stroke pathology, the potential of L-glutamine in the treatment of ischemic stroke is worth exploring.

Aims

In this study, we investigated the effect and mechanisms of action of L-glutamine after cerebral ischemic injury.

Results

L-glutamine reduced brain infarct volume and promoted neurobehavioral recovery in mice. L-glutamine administration increased the expression of heat-shock protein 70 (HSP70) in astrocytes and endothelial cells. Such effects were abolished by the coadministration of Apoptozole, an inhibitor of the ATPase activity of HSP70. L-glutamine also reduced oxidative stress and neuronal apoptosis, and increased the level of superoxide dismutase, glutathione, and brain-derived neurotrophic factor. Cotreatment with Apoptozole abolished these effects. Cell culture study further revealed that the conditioned medium from astrocytes cultured with L-glutamine reduced the apoptosis of neurons after oxygen-glucose deprivation.

Conclusion

L-glutamine attenuated ischemic brain injury and promoted functional recovery via HSP70, suggesting its potential in ischemic stroke therapy.

SUBMITTER: Luo LL

PROVIDER: S-EPMC6698979 | biostudies-literature |

REPOSITORIES: biostudies-literature

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