Ontology highlight
ABSTRACT:
SUBMITTER: Windsor IW
PROVIDER: S-EPMC6699097 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Windsor Ian W IW Graff Crystal J CJ Raines Ronald T RT
Protein science : a publication of the Protein Society 20190806 9
The endogenous production of enzymes as zymogens provides a means to control catalytic activities. Here, we describe the heterologous production of ribonuclease 1 (RNase 1), which is the most prevalent secretory ribonuclease in humans, as a zymogen. In folded RNase 1, the N and C termini flank the enzymic active site. By using intein-mediated cis-splicing, we created circular proteins in which access to the active site of RNase 1 is obstructed by an amino-acid sequence that is recognized by the ...[more]