Dataset Information

Rapid diagnosis of nitrogen status in rice based on Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS).

ABSTRACT: Background:An effective and expeditious approach to assess plant nitrogen status is urgently needed in rice production and management as the conventional chemical methods are laborious and time-consuming. Results:Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was used to record the spectra of rice leaves for the effective diagnosis of nitrogen nutrition status. The band in the wavenumber range of 1680 to 1630 cm-1 was associated with amide I and that from 1570 to 1510 cm-1 with amide II. We attempted to use this information to characterize the nitrogen status in rice plants at different growth stages. The ratio of photoacoustic intensity of amide II to amide I was measured and applied as nitrogen status index, and considering the yields, the ratio showed a positive linear correlation (R2?=?0.9) with the total nitrogen of rice leaves. The ratio at the tillering and full panicle stages were more suitable for diagnosis, a ratio of 0.4-0.55 indicated an adequate nitrogen status, ratios lower than 0.4 indicated a poor nitrogen status; whereas ratios greater than 0.55 indicated excessive nitrogen supply. Conclusion:Our study provides an effective and rapid strategy for nitrogen-supply assessment in rice based on FTIR-PAS, which can guide rational fertilization in rice production.

SUBMITTER: Wu K

PROVIDER: S-EPMC6699123 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Rapid diagnosis of nitrogen status in rice based on Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS).

Wu Ke K Du Changwen C Ma Fei F Shen Yazhen Y Liang Dong D Zhou Jianmin J

Plant methods 20190819

<h4>Background</h4>An effective and expeditious approach to assess plant nitrogen status is urgently needed in rice production and management as the conventional chemical methods are laborious and time-consuming.<h4>Results</h4>Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was used to record the spectra of rice leaves for the effective diagnosis of nitrogen nutrition status. The band in the wavenumber range of 1680 to 1630 cm<sup>-1</sup> was associated with amide I and that f ...[more]

PMID: 31452670

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Project description:Fourier transform infrared (FTIR) spectroscopy, a technology traditionally used in chemistry to determine the molecular composition of a wide range of sample types, has gained growing interest in microbial typing. It is based on the different vibrational modes of the covalent bonds between atoms of a given sample, as bacterial cells, induced by the absorption of infrared radiation. This technique has been largely used for the study of pathogenic species, especially in the clinical field, and has been proposed also for the typing at different subspecies levels. The high throughput, speed, low cost, and simplicity make FTIR spectroscopy an attractive technique also for industrial applications, in particular, for probiotics. The aim of this study was to compare FTIR spectroscopy with established genotyping methods, pulsed-field gel electrophoresis (PFGE), whole-genome sequencing (WGS), and multilocus sequence typing (MLST), in order to highlight the FTIR spectroscopy potential discriminatory power at strain level. Our study focused on bifidobacteria, an important group of intestinal commensals generally recognized as probiotics. For their properties in promoting and maintaining health, bifidobacteria are largely marketed by the pharmaceutical, food, and dairy industries. Strains belonging to Bifidobacterium longum subsp. longum and Bifidobacterium animalis subsp. lactis were taken into consideration together with some additional type strains. For B. longum subsp. longum, it was possible to discriminate the strains with all the methods used. Although two isolates were shown to be strictly phylogenetically related, constituting a unique cluster, based on PFGE, WGS, and MLST, no clustering was observed with FTIR. For B. animalis subsp. lactis group, PFGE, WGS, and MLST were non-discriminatory, and only one strain was easily distinguished. On the other hand, FTIR discriminated all the isolates one by one, and no clustering was observed. According to these results, FTIR analysis is not only equivalent to PFGE, WGS, and MLST, but also for some strains, in particular, for B. animalis subsp. lactis group, more informative, being able to differentiate strains not discernible with the other two methods based on phenotypic variations likely deriving from certain genetic changes. Fourier transform infrared spectroscopy has highlighted the possibility of using the cell surface as a kind of barcode making tracing strains possible, representing an important aspect in probiotic applications. Furthermore, this work constitutes the first investigation on bifidobacterial strain typing using FTIR spectroscopy.

Dataset Information

Rapid diagnosis of nitrogen status in rice based on Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS).

Publications

Rapid diagnosis of nitrogen status in rice based on Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS).

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