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Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging.

ABSTRACT: The simultaneous utilization of electrophysiological recordings and two-photon imaging allows the observation of neural activity in a high temporal and spatial resolution at the same time. The three dimensional monitoring of morphological features near the microelectrode array makes the observation more precise and complex. In vitro experiments were performed on mice neocortical slices expressing the GCaMP6 genetically encoded calcium indicator for monitoring the neural activity with two-photon microscopy around the implanted microelectrodes. A special filtering algorithm was used for data analysis to eliminate the artefacts caused by the imaging laser. Utilization of a special filtering algorithm allowed us to detect and sort single unit activities from simultaneous two-photon imaging and electrophysiological measurement.

SUBMITTER: Orban G

PROVIDER: S-EPMC6701834 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging.

Orbán Gábor G Meszéna Domokos D Tasnády Kinga Réka KR Rózsa Balázs B Ulbert István I Márton Gergely G

PloS one 20190820 8

The simultaneous utilization of electrophysiological recordings and two-photon imaging allows the observation of neural activity in a high temporal and spatial resolution at the same time. The three dimensional monitoring of morphological features near the microelectrode array makes the observation more precise and complex. In vitro experiments were performed on mice neocortical slices expressing the GCaMP6 genetically encoded calcium indicator for monitoring the neural activity with two-photon ...[more]

PMID: 31430357

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Dataset Information

Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging.

Publications

Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging.

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