Comparative anti-osteoporotic properties of the leaves and roots of Marantodes pumilum var. alata in postmenopausal rat model.
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ABSTRACT:
SUBMITTER: Giaze TR
PROVIDER: S-EPMC6702132 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
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Comparative anti-osteoporotic properties of the leaves and roots of <i>Marantodes pumilum var. alata</i> in postmenopausal rat model.
Giaze Tijjani Rabiu TR Shuid Ahmad Nazrun AN Soelaiman Ima Nirwana IN Muhammad Norliza N Jamal Jamia Azdina JA Fauzi Mh Busra MB Mohamed Norazlina N
Journal of traditional and complementary medicine 20190104 4
<h4>Background</h4><i>Marantodes pumilum</i> var. <i>alata</i> (<i>MPva</i>), popularly known as Kacip Fatimah, is widely used to maintain female reproductive health, facilitate post-partum recovery and manage symptoms of menopause and osteoporosis in South-East Asia. This study aims to further evaluate the osteoprotective potential of <i>MPva</i> in view of reports of its bone-protective properties in postmenopausal condition.<h4>Methods</h4>Thirty female Sprague-Dawley rats were sorted into 5 ...[more]
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Project description:Phytoestrogens have attracted considerable attention for their potential in the prevention of postmenopausal osteoporosis. Recently, a phytoestrogen-rich herbal plant, Marantodes pumilum var. alata (Blume) Kuntze was reported to protect against bone loss in ovariectomized rat. However, the bioactive compound responsible for these effects and the underlying mechanism were not known. Through bioassay-guided isolation, demethylbelamcandaquinone B (Dmcq B) was isolated and identified from Marantodes pumilum var. alata leaf extract. In terms of its bone anabolic effects, Dmcq B was at par with 17β-estradiol (E2), in promoting the proliferation, differentiation and mineralization of osteoblast cells. Dmcq-B increased early differentiation markers, collagen content and enzymatic ALP activity. It was demonstrated to regulate BMP2 signaling pathway which further activated the transcription factor, osterix. Subsequently, Dmcq B was able to increase the osteocalcin expression which promoted matrix mineralization as evidenced by the increase in calcium deposition. Dmcq B also reduced the protein level of receptor activator of NF-κβ ligand (RANKL) and promoted osteoprotegerin (OPG) protein expression by osteoblast cells, therefore hastening bone formation rate by decreasing RANKL/OPG ratio. Moreover, Dmcq B was able to increase ER expression, postulating its phytoestrogen property. As the conclusion, Dmcq B is the active compound isolated from Marantodes pumilum var. alata leaves, regulating osteoanabolic activities potentially through the BMP2 and ER signaling pathways.
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