Dataset Information

Identification of novel Glutathione S-Transferases epsilon 2 mutation in Anopheles maculipennis s.s. (Diptera: Culicidae).

ABSTRACT: Anopheles maculipennis complex comprises some important malaria vectors in Iran, Middle East, and Europ. The principal way to control of malaria remains on the use of chemical insecticides against its vectors because there is no vaccine for malaria prevention. Extensive use of organophosphate compounds has caused to emergence and distribution of insecticide resistance in Anopheles species in Asia. The current study aimed to the detection of three well-known amino acid substitutions (I114T, L119F, and F120L) in the Glutathione S-Transferases epsilon 2 (GSTe2) gene are associated with DDT and organophosphate insecticides resistance in an Anopheles maculipennis population collected from Iran. Adult samples of An. maculipennis were collected by hand and Total catch in Animal and Human Shelters from Azerbaijan-Gharbi and Zanjan provinces. Following morphological identification, DNA was extracted by YTA Genomic DNA Extraction Mini Kit for amplification of rDNA-ITS2 and GSTe2 fragments. ?500 bp fragment was amplified using F rDNA-ITS2 and GSTe2 primers. rDNA-ITS2 sequence analysis showed 100% similarity with An. maculipennis. GSTe2 nucleotide sequence similarity within species was 99-100%, while, it was 95-96 % when compared with Anopheles sacharovi GSTe2 sequences available in GenBank. Amino acid sequence comparisons showed a novel amino acid substitution in N148D position with 15.79% frequency. The current study reports new GSTe2 amino acid substitution in An. maculipennis s.s., for the first time. The function of the mutation N148D and its association with resistance phenotype need to validate. However, the integration of these data into the malaria control program still remains a challenge.

SUBMITTER: Asadi Saatlou Z

PROVIDER: S-EPMC6702443 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Identification of novel Glutathione S-Transferases epsilon 2 mutation in Anopheles maculipennis s.s. (Diptera: Culicidae).

Asadi Saatlou Zahra Z Sedaghat Mohammad Mehdi MM Taghilou Behrooz B Gholizadeh Saber S

Heliyon 20190812 8

Anopheles maculipennis complex comprises some important malaria vectors in Iran, Middle East, and Europ. The principal way to control of malaria remains on the use of chemical insecticides against its vectors because there is no vaccine for malaria prevention. Extensive use of organophosphate compounds has caused to emergence and distribution of insecticide resistance in Anopheles species in Asia. The current study aimed to the detection of three well-known amino acid substitutions ...[more]

PMID: 31453404

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Project description:BackgroundDetermining the host feeding patterns of mosquitoes by identifying the origin of their blood-meals is an important part of understanding the role of vector species in current and future disease transmission cycles. Collecting large numbers of blood-fed mosquitoes from the field is difficult, therefore it is important to maximise the information obtained from each specimen. This study aimed to use mosquito genome sequence to identify the species within Anopheles maculipennis sensu lato (An. maculipennis s.l.), identify the vertebrate hosts of field-caught blood-fed An. maculipennis s.l. , and to test for the presence of myxoma virus (Poxviridae, genus Leporipoxvirus) in specimens found to have fed on the European rabbit (Oryctolagus cuniculus).MethodsBlood-fed An. maculipennis s.l. were collected from resting sites at Elmley Nature Reserve, Kent, between June and September 2013. Hosts that An. maculipennis s.l. had fed on were determined by a PCR-sequencing approach based on the partial amplification of the mitochondrial cytochrome C oxidase subunit I gene. Mosquitoes were then identified to species by sequencing a region of the internal transcribed spacer-2. DNA extracts from all mosquitoes identified as having fed on rabbits were subsequently screened using PCR for the presence of myxoma virus.ResultsA total of 94 blood-fed Anopheles maculipennis s.l. were collected, of which 43 (46%) provided positive blood-meal identification results. Thirty-six of these specimens were identified as Anopheles atroparvus, which had fed on rabbit (n = 33, 92%) and cattle (n = 3, 8%). Seven mosquitoes were identified as Anopheles messeae, which had fed on cattle (n = 6, 86%) and dog (n = 1, 14%). Of the 33 An. atroparvus that contained rabbit blood, nine (27%) were positive for myxoma virus.ConclusionsResults demonstrate that a single DNA extract from a blood-fed mosquito can be successfully used for molecular identification of members of the An. maculipennis complex, blood-meal identification, and for the targeted detection of a myxoma virus. This study shows that An. atroparvus has a strong feeding preference for both healthy and myxoma-infected rabbits, providing evidence that this species may play a significant role in the transmission of myxomatosis among wild rabbit populations in the United Kingdom (UK).

Dataset Information

Identification of novel Glutathione S-Transferases epsilon 2 mutation in Anopheles maculipennis s.s. (Diptera: Culicidae).

Publications

Identification of novel Glutathione S-Transferases epsilon 2 mutation in <i>Anopheles maculipennis</i> s.s. (Diptera: Culicidae).

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