Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India.
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ABSTRACT: Aim
The present study was carried out to find out the causative agent of exanthematous skin lesions in sheep maintained by Southern Regional Research Centre, Mannavanur, Kodai hills, Tamil Nadu.Materials and methods
Polymerase chain reaction (PCR) with Orf virus (ORFV) B2L gene-specific primers was carried out by employing the total genomic DNA isolated from the scabs as the template. The ORFV isolates from Kodai hills were characterized by the use of bioinformatics tools.Results
The amino acid identity of ORFV isolate 1 from Kodai hills is having 98.14%, 96.29%, and 83.59% identity with reference strains of ORFV, Pseudocowpox virus, and bovine papular stomatitis virus, respectively. Phylogenetic analysis revealed that ORFV isolates from Kodai hills clustered with the other ORFV isolates from different geographical areas of India.Conclusion
The etiological agent of exanthematous skin lesion among sheep of Kodai hills is ORFV.
SUBMITTER: Nagarajan G
PROVIDER: S-EPMC6702573 | biostudies-literature |
REPOSITORIES: biostudies-literature
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