Delivery of siRNA therapeutics using cowpea chlorotic mottle virus-like particles.

ABSTRACT: While highly promising in medicine, gene therapy requires delivery agents to protect and target nucleic acid therapeutics. We developed a plant viral siRNA delivery platform making use of self-assembling cowpea chlorotic mottle virus (CCMV). CCMV was loaded with siRNAs targeting GFP or FOXA1; to further enhance cell uptake and intracellular trafficking, resulting in more efficient gene knockdown, we appended CCMV with a cell penetrating peptide (CPP), specifically M-lycotoxin peptide L17E.

SUBMITTER: Lam P 

PROVIDER: S-EPMC6705399 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature