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Delivery of siRNA therapeutics using cowpea chlorotic mottle virus-like particles.


ABSTRACT: While highly promising in medicine, gene therapy requires delivery agents to protect and target nucleic acid therapeutics. We developed a plant viral siRNA delivery platform making use of self-assembling cowpea chlorotic mottle virus (CCMV). CCMV was loaded with siRNAs targeting GFP or FOXA1; to further enhance cell uptake and intracellular trafficking, resulting in more efficient gene knockdown, we appended CCMV with a cell penetrating peptide (CPP), specifically M-lycotoxin peptide L17E.

SUBMITTER: Lam P 

PROVIDER: S-EPMC6705399 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Delivery of siRNA therapeutics using cowpea chlorotic mottle virus-like particles.

Lam Patricia P   Steinmetz Nicole F NF  

Biomaterials science 20190701 8


While highly promising in medicine, gene therapy requires delivery agents to protect and target nucleic acid therapeutics. We developed a plant viral siRNA delivery platform making use of self-assembling cowpea chlorotic mottle virus (CCMV). CCMV was loaded with siRNAs targeting GFP or FOXA1; to further enhance cell uptake and intracellular trafficking, resulting in more efficient gene knockdown, we appended CCMV with a cell penetrating peptide (CPP), specifically M-lycotoxin peptide L17E. ...[more]

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