Ontology highlight
ABSTRACT:
SUBMITTER: Alizadeha AA
PROVIDER: S-EPMC6706722 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Alizadeha Ali Akbar AA Hamzeh-Mivehroud Maryam M Haddad Elnaz E Haddad Nazanin N Sharifi Mehdi M Mohammadi Samin S Pourtaghi-Anvarian Samira S Dastmalchi Siavoush S
Iranian journal of pharmaceutical research : IJPR 20190101 2
Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine which plays crucial roles in pathogenesis of inflammatory diseases. The current study aimed to investigate the binding abilities of I44 and I49 domain antibodies to TNF-α. The dAbs were expressed in bacterial expression system and purified by affinity chromatography using Ni-sepharose column. The expression and purity of the proteins were evaluated using western blotting and SDS-PAGE techniques, respectively. ELISA experiment showed ...[more]